Ratiometric Temperature Sensing with Semiconducting Polymer Dots

This communication describes ultrabright single-nanoparticle ratiometric temperature sensors based on semiconducting polymer dots (Pdots). We attached the temperature sensitive dyeRhodamine B (RhB), whose emission intensity decreases with increasing temperaturewithin the matrix of Pdots. The as-pr...

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Published inJournal of the American Chemical Society Vol. 133; no. 21; pp. 8146 - 8149
Main Authors Ye, Fangmao, Wu, Changfeng, Jin, Yuhui, Chan, Yang-Hsiang, Zhang, Xuanjun, Chiu, Daniel T
Format Journal Article
LanguageEnglish
Published United States American Chemical Society 01.06.2011
Subjects
Energy Transfer
Fluorescent Dyes
Nanoparticles
Nanotechnology - methods
Polymers
Rhodamines - chemistry
Semiconductors
Temperature
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Summary:This communication describes ultrabright single-nanoparticle ratiometric temperature sensors based on semiconducting polymer dots (Pdots). We attached the temperature sensitive dyeRhodamine B (RhB), whose emission intensity decreases with increasing temperaturewithin the matrix of Pdots. The as-prepared Pdot-RhB nanoparticle showed excellent temperature sensitivity and high brightness because it took advantage of the light harvesting and amplified energy transfer capability of Pdots. More importantly, the Pdot-RhB nanoparticle showed ratiometric temperature sensing under a single wavelength excitation and has a linear temperature sensing range that matches well with the physiologically relevant temperatures. We employed Pdot-RhB for measuring intracellular temperatures in a live-cell imaging mode. The exceptional brightness of Pdot-RhB allows this nanoscale temperature sensor to be used also as a fluorescent probe for cellular imaging.
ISSN:0002-7863
1520-5126
1520-5126
DOI:10.1021/ja202945g