Smart Hydrogels Containing Adenylate Kinase: Translating Substrate Recognition into Macroscopic Motion

Enzyme-based hybrid hydrogels were prepared by covalently incorporating an adenylate kinase mutant, possessing two thiol groups, into HPMA copolymer- or PEG-based hydrogel structures. The nanoscale conformational change of enzyme, triggered by substrate recognition, translated into macroscopic motio...

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Bibliographic Details
Published inJournal of the American Chemical Society Vol. 130; no. 47; pp. 15760 - 15761
Main Authors Yuan, Weiwei, Yang, Jiyuan, Kopečková, Pavla, Kopeček, Jindřich
Format Journal Article
LanguageEnglish
Published United States American Chemical Society 26.11.2008
Subjects
Adenylate Kinase - chemistry
Adenylate Kinase - genetics
Adenylate Kinase - metabolism
Escherichia coli - enzymology
Escherichia coli - genetics
Hydrogels - chemistry
Models, Molecular
Molecular Structure
Protein Biosynthesis
Substrate Specificity
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Summary:Enzyme-based hybrid hydrogels were prepared by covalently incorporating an adenylate kinase mutant, possessing two thiol groups, into HPMA copolymer- or PEG-based hydrogel structures. The nanoscale conformational change of enzyme, triggered by substrate recognition, translated into macroscopic motion of hydrogels.
Bibliography:ark:/67375/TPS-B570FD52-2
Experimental details of the synthesis, purification and enzymatic activity of AKtm, the synthesis of hydrogels, the labeling of AKtm, and swelling. This material is available free of charge via the Internet at http://pubs.acs.org.
istex:5CF1882791DFD2A06C51DDA5CE1E42BB06425DA6
ObjectType-Article-1
SourceType-Scholarly Journals-1
ObjectType-Feature-2
content type line 23
ISSN:0002-7863
1520-5126
DOI:10.1021/ja805634x