Plasmonic Gold Nanostar-Enhanced Multimodal Photoacoustic Microscopy and Optical Coherence Tomography Molecular Imaging To Evaluate Choroidal Neovascularization

Although photoacoustic microscopy (PAM) and optical coherence tomography (OCT) allow visualization of the retinal microvasculature, distinguishing early neovascularization from adjacent vessels remains challenging. Herein, gold nanostars (GNSs) functionalized with an RGD peptide were utilized as mul...

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Published inACS sensors Vol. 5; no. 10; pp. 3070 - 3081
Main Authors Nguyen, Van-Phuc, Li, Yanxiu, Henry, Jessica, Zhang, Wei, Aaberg, Michael, Jones, Sydney, Qian, Thomas, Wang, Xueding, Paulus, Yannis M
Format Journal Article
LanguageEnglish
Published United States American Chemical Society 23.10.2020
Subjects
Animals
Choroidal Neovascularization
Gold
Microscopy
Molecular Imaging
Photoacoustic Techniques
Rabbits
Tomography, Optical Coherence
gold nanostars
photoacoustic microscopy
photoacoustic ophthalmoscopy
optical coherence tomography
choroidal neovascularization
contrast agents
multimodal molecular imaging
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Summary:Although photoacoustic microscopy (PAM) and optical coherence tomography (OCT) allow visualization of the retinal microvasculature, distinguishing early neovascularization from adjacent vessels remains challenging. Herein, gold nanostars (GNSs) functionalized with an RGD peptide were utilized as multimodality contrast agents for both PAM and OCT. GNSs have great absorption and scattering characteristics in the near-infrared region where most vasculature and tissue generates a less intrinsic photoacoustic signal while having a small size, excellent biocompatibility in vivo, and great photostability under nanosecond pulsed laser illumination. This enabled visualization and differentiation of individual microvasculature in vivo using multimodal PAM and OCT imaging. Detailed three-dimensional imaging of GNSs was achieved in an important choroidal neovascularization model in living rabbits. Through the administration of GNSs, PA contrast increased up to 17-fold and OCT intensities increased 167%. This advanced molecular-imaging platform with GNSs provides a unique tool for detailed mapping of the pathogenesis of the microvasculature.
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V.P.N., planned, designed, and performed the experiments, employed animal work, maintenance the imaging system, data analysis, in vitro characterization and in vivo OCT and PAM experiments, reconstructed three-dimensional visualization using Amira, and prepared of the manuscript. Y.L. carried out animal model. J.H., M.A. and S. J. contributed to histological analysis, and in vitro phantom experiments. W.Z assisted with maintenance of the imaging system. X. W. was responsible for experimental designs, overall evaluation of the data and writing of the manuscript. Y.M.P directed the project, organized and wrote the manuscript. All authors performed a critical evaluation of the data, experimental planning, data analysis, and critical revision of the manuscript.
Author Contributions
ISSN:2379-3694
2379-3694
DOI:10.1021/acssensors.0c00908