Ellagitannin Composition of Blackberry As Determined by HPLC-ESI-MS and MALDI-TOF-MS
Blackberries (Rubus sp.) were evaluated by high-performance liquid chromatography−electrospray ionization−mass spectrometry (HPLC-ESI-MS) and matrix-assisted laser desorption/ionization−time-of-flight mass spectrometry (MALDI-TOF-MS) to identify the ellagitannins present in flesh, torus (receptacle...
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Published in | Journal of agricultural and food chemistry Vol. 56; no. 3; pp. 661 - 669 |
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Main Authors | , , , , |
Format | Journal Article |
Language | English |
Published |
United States
American Chemical Society
13.02.2008
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Subjects | |
Online Access | Get full text |
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Summary: | Blackberries (Rubus sp.) were evaluated by high-performance liquid chromatography−electrospray ionization−mass spectrometry (HPLC-ESI-MS) and matrix-assisted laser desorption/ionization−time-of-flight mass spectrometry (MALDI-TOF-MS) to identify the ellagitannins present in flesh, torus (receptacle tissue), and seeds. Most ellagitannins were present (or detectable) only in seed tissues. Ellagitannins identified by HPLC-ESI-MS in the seeds included pedunculagin, casuarictin/potentillin, castalagin/vescalagin, lambertianin A/sanguiin H-6, lambertianin C, and lambertianin D. For several of the ellagitannins, isomeric separation was also obtained. The MALDI-TOF-MS analysis was primarily utilized to evaluate and identify high molecular mass (>1000 Da) ellagitannins. The MALDI analysis verified the presence of the ellagitannins identified by HPLC-ESI-MS including lambertianin A/sanguiin H-6, lambertianin C, and lambertianin D, but the analysis also indicated the presence of several other compounds that were most likely ellagitannins based on the patterns observed in the masses (i.e., loss or addition of a gallic acid moiety to a known ellagitannin). This study determined the presence of several possible isomeric forms of ellagitannins previously unidentified in fruit and presents a possible analytical HPLC method for the analysis of the major ellagitannins present in the fruit. |
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Bibliography: | http://hdl.handle.net/10113/18087 http://dx.doi.org/10.1021/jf071990b Mention of a trade name, proprietary product, or specific equipment does not constitute a guarantee by the U.S. Department of Agriculture and does not imply its approval to the exclusion of other products that may be suitable. istex:3FCDB8626A79F503D8CBD0943DF18380C5E1E4C6 ark:/67375/TPS-2TBPZQ93-Z ObjectType-Article-1 SourceType-Scholarly Journals-1 ObjectType-Feature-2 content type line 23 |
ISSN: | 0021-8561 1520-5118 |
DOI: | 10.1021/jf071990b |