Nanoparticle Probes with Surface Enhanced Raman Spectroscopic Tags for Cellular Cancer Targeting

• Published in: Analytical chemistry (Washington) Vol. 78; no. 19; pp. 6967 - 6973
• Main Authors: Kim, Jong-Ho, Kim, Jun-Sung, Choi, Heejeong, Lee, Sang-Myung, Jun, Bong-Hyun, Yu, Kyeong-Nam, Kuk, Eunye, Kim, Yong-Kweon, Jeong, Dae Hong, Cho, Myung-Haing, Lee, Yoon-Sik
• Format: Journal Article
• Language: English
• Published: Washington, DC American Chemical Society 01.10.2006
• Subjects: Analytical chemistry; Cancer; Chemistry; Exact sciences and technology; Humans; Medical screening; Metal Nanoparticles; Nanoparticles; Neoplasms - pathology; Silica; Spectrometric and optical methods; Spectrum analysis; Spectrum Analysis, Raman - methods; Multiplexing; Silver; Antibody; Nanoparticle; Surface Enhanced Raman Spectrometry; Membrane; Raman spectrometry; Silica; Tumor cell; Quantitative analysis
• ISSN: 0003-2700; 1520-6882
• DOI: 10.1021/ac0607663

We have developed biocompatible, photostable, and multiplexing-compatible surface-enhanced Raman spectroscopic tagging material (SERS dots) composed of silver nanoparticle-embedded silica spheres and organic Raman labels for cellular cancer targeting in living cells. SERS dots showed linear dependency of Raman signatures on their different amounts, allowing their possibility for the quantification of targets. In addition, the antibody-conjugated SERS dots were successfully applied to the targeting of HER2 and CD10 on cellular membranes and exhibited good specificity. SERS dots demonstrate the potential for high-throughput screening of biomolecules using vibrational information.