Dual Fluorescence Sensor for Trace Oxygen and Temperature with Unmatched Range and Sensitivity

• Published in: Analytical chemistry (Washington) Vol. 80; no. 16; pp. 6449 - 6457
• Main Authors: Baleizão, Carlos, Nagl, Stefan, Schäferling, Michael, Berberan-Santos, Mário N, Wolfbeis, Otto S
• Format: Journal Article
• Language: English
• Published: Washington, DC American Chemical Society 15.08.2008
• Subjects: Analytical chemistry; Biosensing Techniques; Calibration; Chemistry; Exact sciences and technology; Fluorescence; Fluorescent Dyes - chemistry; Fullerenes - chemistry; General, instrumentation; High temperature; Luminescence; Luminescent Measurements; Optics and Photonics - instrumentation; Oxygen; Oxygen - chemistry; Phenanthrolines - chemistry; Polymer films; Polymers - chemistry; Ruthenium - chemistry; Sensitivity and Specificity; Sensors; Spectrometric and optical methods; Studies; Temperature; Trace analysis; Oxygen; Dyes; Ruthenium; Acrylonitrile; Cellulose; Optical sensor; Temperature sensor; Polymer; Foodstuff; High temperature; Chemical sensor; Leak detection; Fluorescence spectrometry; Lifetime; Sensitivity; Response time; Imaging; Detection limit; Fullerenes; Phenanthrolines; Luminescence spectrometry; Monitoring
• ISSN: 0003-2700; 1520-6882
• DOI: 10.1021/ac801034p

An optical dual sensor for oxygen and temperature is presented that is highly oxygen sensitive and covers a broad temperature range. Dual sensing is based on luminescence lifetime measurements. The novel sensor contains two luminescent compounds incorporated into polymer films. The temperature-sensitive dye (ruthenium tris-1,10-phenanthroline) has a highly temperature-dependent luminescence and is incorporated in poly(acrylonitrile) to avoid cross-sensitivity to oxygen. Fullerene C70 was used as the oxygen-sensitive probe owing to its strong thermally activated delayed fluorescence at elevated temperatures that is extremely oxygen sensitive. The cross-sensitivity of C70 to temperature is accounted for by means of the temperature sensor. C70 is incorporated into a highly oxygen-permeable polymer, either ethyl cellulose or organosilica. The two luminescent probes have different emission spectra and decay times, and their emissions can be discriminated using both parameters. Spatially resolved sensing is achieved by means of fluorescence lifetime imaging. The response times of the sensor to oxygen are short. The dual sensor exhibits a temperature operation range between at least 0 and 120 °C, and detection limits for oxygen in the ppbv range, operating for oxygen concentrations up to at least 50 ppmv. These ranges outperform all dual oxygen and temperature sensors reported so far. The dual sensor presented in this study is especially appropriate for measurements under extreme conditions such as high temperatures and ultralow oxygen levels. This dual sensor is a key step forward in a number of scientifically or commercially important applications including food packaging, for monitoring of hyperthermophilic microorganisms, in space technology, and safety and security applications in terms of detection of oxygen leaks.