Effects of a Chitin-Binding Vicilin from Enterolobium contortisiliquum Seeds on Bean Bruchid Pests (Callosobruchus maculatus and Zabrotes subfasciatus) and Phytopathogenic Fungi (Fusarium solani and Colletrichum lindemuntianum)

• Published in: Journal of agricultural and food chemistry Vol. 55; no. 2; pp. 260 - 266
• Main Authors: Moura, Fabiano T, Oliveira, Adeliana S, Macedo, Leonardo L. P, Vianna, André L. B. R, Andrade, Lucia B. S, Martins-Miranda, A. S, Oliveira, Jose T. A, Santos, Elizeu A, de Sales, Mauricio P
• Format: Journal Article
• Language: English
• Published: Washington, DC American Chemical Society 24.01.2007
• Subjects: Animals; antifungal properties; binding capacity; Biological and medical sciences; Callosobruchus maculatus; chitin; Chitin - metabolism; chitin-binding vicilin; Coleoptera; Colletotrichum lindemuthianum; Enterolobium contortisiliquum; Fabaceae - chemistry; Food industries; Fruit and vegetable industries; Fundamental and applied biological sciences. Psychology; Fungicides, Industrial - isolation & purification; Fungicides, Industrial - pharmacology; Fusarium solani; insecticidal properties; Insecticides - isolation & purification; larvae; molecular weight; mortality; Plant Proteins - isolation & purification; Plant Proteins - pharmacology; Seed Storage Proteins; seeds; Seeds - chemistry; spore germination; vicilin; Zabrotes subfasciatus; Binding; Vegetables; Seeds; Plant pathogen; Coleoptera; Insecta; Chitin; chitin binding; Callosobruchus maculatus; Bean; Fungi; Zabrotes subfasciatus; Vicilin; bean bruchid pests; Pest; Arthropoda; Bruchidae; Fusarium solani; Fungi Imperfecti; Invertebrata; anti-insect activity; Thallophyta; phytopathogenic fungi
• ISSN: 0021-8561; 1520-5118
• DOI: 10.1021/jf061623k

Chitin-binding vicilin from Enterolobium contortisiliquum seeds was purified by ammonium sulfate followed by gel filtration on Sephacryl 300-SH and on Sephacryl 200-SH. The vicilin, called EcV, is a dimeric glycoprotein composed of 1.03% carbohydrates and a M r of 151 kDa, consisting of two subunits of M r of 66.2 and 63.8 kDa. The EcV homogeneity was confirmed in a PAGE where it was observed to be a unique acid protein band with slow mobility in this native gel. E. contortisiliquum vicilin (EcV) was tested for anti-insect activity against C. maculatus and Zabrotes subfasciatus larvae and for phytopathogenic fungi, F. solani and C. lindemuntianum. EcV was very effective against both bruchids, producing 50% mortality for Z. subfasciatus at an LD50 of 0.43% and affected 50% of the larvae mass with an ED50 of 0.65%. In artificial diets given to C. maculatus, 50% of the larvae mass was affected with an ED50 of 1.03%, and larva mortality was 50% at LD50 of 1.11%. EcV was not digested by midgut homogenates of C. maculatus and Z. Subfasciatus until 12 h of incubation, and at 24 h EcV was more resistant to Z. subfasciatus larval proteases. The binding to chitin present in larvae gut associated to low EcV digestibility could explain its lethal effects. EcV also exerted an inhibitory effect on the germination of F. solani at concentrations of 10 and 20 μg mL−1. The effect of EcV on fungi is possibly due to binding to chitin-containing structures of the fungal cell wall. Keywords: Vicilin; bean bruchid pests; chitin binding; phytopathogenic fungi; anti-insect activity