Applying improved ddPCR to reliable quantification of MPXV in clinical settings

• Published in: Microbiology spectrum Vol. 12; no. 7; p. e0001824
• Main Authors: Liang, Chudan, Yang, Huiqin, Yang, Xiaofeng, Long, Zhenyu, Zhou, Yuandong, Wang, Jian, Fan, Linjin, Zeng, Mou, Wang, Yulong, Zheng, Haipeng, Wang, Zequn, Ye, Pengfei, Lin, Jingyan, Shi, Wendi, Huang, Hongxin, Yan, Huijun, Qian, Jun, Li, Linghua, Liu, Linna
• Format: Journal Article
• Language: English
• Published: United States American Society for Microbiology 02.07.2024
• Subjects: Adult; China; Clinical Microbiology; ddPCR; Disease Outbreaks; Feces - virology; Female; Humans; Male; monkeypox virus; Monkeypox virus - genetics; Monkeypox virus - isolation & purification; Mpox; Mpox (monkeypox) - diagnosis; Mpox (monkeypox) - virology; Polymerase Chain Reaction - methods; quantification; Real-Time Polymerase Chain Reaction - methods; Research Article; Sensitivity and Specificity; Viral Load - methods; viral loads; China; ddPCR; viral loads; Mpox; quantification; monkeypox virus

The ddPCR technique proved to be a sensitive and valuable tool for accurately quantifying MPXV viral loads in various clinical specimen types. The findings provided valuable insights into the necessary pre-treatment protocols for MPXV diagnosis in ddPCR detection and the potentially suitable sample...