Applying improved ddPCR to reliable quantification of MPXV in clinical settings

The ddPCR technique proved to be a sensitive and valuable tool for accurately quantifying MPXV viral loads in various clinical specimen types. The findings provided valuable insights into the necessary pre-treatment protocols for MPXV diagnosis in ddPCR detection and the potentially suitable sample...

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Published inMicrobiology spectrum Vol. 12; no. 7; p. e0001824
Main Authors Liang, Chudan, Yang, Huiqin, Yang, Xiaofeng, Long, Zhenyu, Zhou, Yuandong, Wang, Jian, Fan, Linjin, Zeng, Mou, Wang, Yulong, Zheng, Haipeng, Wang, Zequn, Ye, Pengfei, Lin, Jingyan, Shi, Wendi, Huang, Hongxin, Yan, Huijun, Qian, Jun, Li, Linghua, Liu, Linna
Format Journal Article
LanguageEnglish
Published United States American Society for Microbiology 02.07.2024
Subjects
Adult
China
Clinical Microbiology
ddPCR
Disease Outbreaks
Feces - virology
Female
Humans
Male
monkeypox virus
Monkeypox virus - genetics
Monkeypox virus - isolation & purification
Mpox
Mpox (monkeypox) - diagnosis
Mpox (monkeypox) - virology
Polymerase Chain Reaction - methods
quantification
Real-Time Polymerase Chain Reaction - methods
Research Article
Sensitivity and Specificity
Viral Load - methods
viral loads
China
ddPCR
viral loads
Mpox
quantification
monkeypox virus
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Summary:The ddPCR technique proved to be a sensitive and valuable tool for accurately quantifying MPXV viral loads in various clinical specimen types. The findings provided valuable insights into the necessary pre-treatment protocols for MPXV diagnosis in ddPCR detection and the potentially suitable sample types for collection. Therefore, such results can aid in comprehending the potential characteristics of MPXV infection and the usage of ddPCR in clinical settings.
Bibliography:ObjectType-Article-1
SourceType-Scholarly Journals-1
ObjectType-Feature-2
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Chudan Liang, Huiqin Yang, Xiaofeng Yang, and Zhenyu Long contributed equally to this article. Author order was determined based on their contributions.
The authors declare no conflict of interest.
ISSN:2165-0497
2165-0497
DOI:10.1128/spectrum.00018-24