High-Density Fiber-Optic DNA Random Microsphere Array

• Published in: Analytical chemistry (Washington) Vol. 72; no. 22; pp. 5618 - 5624
• Main Authors: Ferguson, Jane A, Steemers, Frank J, Walt, David R
• Format: Journal Article
• Language: English
• Published: Washington, DC American Chemical Society 15.11.2000
• Subjects: Base Sequence; Biological and medical sciences; Deoxyribonucleic acid; Diverse techniques; DNA; DNA - chemistry; DNA microarray; Fiber Optic Technology; Fiber optics; Fundamental and applied biological sciences. Psychology; Microspheres; Molecular and cellular biology; Nucleic Acid Hybridization; Oligonucleotide Probes; Optical Fibers; Sensitivity and Specificity; Molecular hybridization; Microsphere; Analysis method; Fluorescent labelling; Immobilization; Oligonucleotide; Molecular probe; Miniaturization; Optical fiber
• ISSN: 0003-2700; 1520-6882
• DOI: 10.1021/ac0008284

A high-density fiber-optic DNA microarray sensor was developed to monitor multiple DNA sequences in parallel. Microarrays were prepared by randomly distributing DNA probe-functionalized 3.1-μm-diameter microspheres in an array of wells etched in a 500-μm-diameter optical imaging fiber. Registration of the microspheres was performed using an optical encoding scheme and a custom-built imaging system. Hybridization was visualized using fluorescent-labeled DNA targets with a detection limit of 10 fM. Hybridization times of seconds are required for nanomolar target concentrations, and analysis is performed in minutes.