Determination of Bacterial Antibiotic Resistance Based on Osmotic Shock Response

We investigate the buoyant mass of bacterial cells in real time with the suspended microchannel resonator (SMR) as the population recovers from an osmotic shock. The density of the culture medium is chosen such that the bacteria initially have a positive buoyant mass which becomes negative as they r...

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Bibliographic Details
Published inAnalytical chemistry (Washington) Vol. 81; no. 16; pp. 7087 - 7090
Main Authors Knudsen, Scott M, von Muhlen, Marcio G, Schauer, David B, Manalis, Scott R
Format Journal Article
LanguageEnglish
Published Washington, DC American Chemical Society 15.08.2009
Subjects
Anti-Bacterial Agents - pharmacology
Applied microbiology
Biological and medical sciences
Cell cultures. Hybridization. Fusion
Citrobacter rodentium - drug effects
Drug Resistance, Microbial
Escherichia coli - drug effects
Fundamental and applied biological sciences. Psychology
Microbiology
Molecular and cellular biology
Osmosis
Osmotic shock
Cell culture
Bacteria
Buoyant density
Real time
Antibiotic resistance
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Summary:We investigate the buoyant mass of bacterial cells in real time with the suspended microchannel resonator (SMR) as the population recovers from an osmotic shock. The density of the culture medium is chosen such that the bacteria initially have a positive buoyant mass which becomes negative as they recover from the hyperosmotic stress. This behavior can be used to differentiate between an antibiotic-resistant and an antibiotic-susceptible strain of the pathogenic bacteria Citrobacter rodentium, and we propose a general approach for exploiting the high precision of the SMR for rapid detection of antibiotic resistance.
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ISSN:0003-2700
1520-6882
DOI:10.1021/ac900968r