Characterization and Antitumor Effect of a Novel Polysaccharide from Grifola frondosa

• Published in: Journal of agricultural and food chemistry Vol. 57; no. 21; pp. 10143 - 10149
• Main Authors: Masuda, Yuki, Matsumoto, Akihisa, Toida, Toshihiko, Oikawa, Tadao, Ito, Koichi, Nanba, Hiroaki
• Format: Journal Article Conference Proceeding
• Language: English
• Published: Washington, DC American Chemical Society 11.11.2009
• Subjects: Animals; anticarcinogenic activity; Antineoplastic Agents - chemistry; Antineoplastic Agents - isolation & purification; Antineoplastic Agents - pharmacology; Bioactive Constituents; Biological and medical sciences; Cell Line, Tumor; Cell Proliferation - drug effects; cell-mediated immunity; Female; Food industries; Fundamental and applied biological sciences. Psychology; Grifola - chemistry; Grifola frondosa; human cell lines; medicinal plants; Meripilaceae; Mice; Mice, Inbred BALB C; Molecular Weight; mushrooms; Neoplasms - drug therapy; Neoplasms - immunology; Neoplasms - physiopathology; polysaccharides; Polysaccharides - chemistry; Polysaccharides - isolation & purification; Polysaccharides - pharmacology; Specific Pathogen-Free Organisms; Xenograft Model Antitumor Assays; immunomodulatory effect; Polysaccharide; Grifola frondosa; antitumor effect; Antineoplastic agent; Characterization; Immunomodulation
• ISSN: 0021-8561; 1520-5118
• DOI: 10.1021/jf9021338

A novel polysaccharide, MZF, with a molecular mass of 23 kDa was isolated from Grifola frondosa. Results from methylation and 1H NMR led to the conclusion that MZF is a heteropolysaccharide consisting of →6)-α-d-Galp-(1→ (36.2%), →3)-α-l-Fucp-(1→ (14.5%), →6)-α-d-Manp-(1→ (9.4%), →3)-β-d-Glcp-(1→ (10.1%), α-d-Manp-(1→ (23.2%), and →3,6)-β-d-Glcp-(1→ (6.5%). Although MZF did not affect the proliferation of colon-26 cells in vitro, it significantly inhibited tumor growth in BALB/cA mice inoculated with colon-26 cancer cells. Moreover, MZF significantly induced the proliferation of splenocytes and peritoneal macrophages. The mRNA expression of IL-12p40, IL-2 and IFN-γ were increased significantly in MZF-treated spleen. Furthermore, MZF augmented the percentage of IFN-γ-producing cells in both splenic CD4+ and CD8+ T cells and tumor infiltrating CD4+ and CD8+ T cells and enhanced the cytotoxic activity of NK cells and CTLs. These results indicate that MZF is a novel effective immunomodulator that has antitumor activity associated with induced cell-mediated immunity.