Control of Protein Orientation on Gold Nanoparticles

Gold nanoparticles (Au NPs) have attracted much attention due to their potential applications in nanomedicine. While numerous studies have quantified biomolecular adsorption to Au NPs in terms of equilibrium binding constants, far less is known about biomolecular orientation on nanoparticle surfaces...

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Published inJournal of physical chemistry. C Vol. 119; no. 36; pp. 21035 - 21043
Main Authors Lin, Wayne, Insley, Thomas, Tuttle, Marcus D, Zhu, Lingyang, Berthold, Deborah A, Král, Petr, Rienstra, Chad M, Murphy, Catherine J
Format Journal Article
LanguageEnglish
Published United States American Chemical Society 10.09.2015
Subjects
protein adsorption
surface chemistry
protein orientation
Gold nanoparticles
α-synuclein
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Summary:Gold nanoparticles (Au NPs) have attracted much attention due to their potential applications in nanomedicine. While numerous studies have quantified biomolecular adsorption to Au NPs in terms of equilibrium binding constants, far less is known about biomolecular orientation on nanoparticle surfaces. In this study, the binding of the protein α-synuclein to citrate and (16-mercapto­hexadecyl)­trimethyl­ammonium bromide (MTAB)-coated 12 nm Au NPs is examined by heteronuclear single quantum coherence NMR spectroscopy to provide site-specific measurements of protein–nanoparticle binding. Molecular dynamics simulations support the orientation assignments, which show N-terminus binding to the Au NP for citrate-capped NPs and C-terminus binding for the MTAB-capped NPs.
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ISSN:1932-7447
1932-7455
DOI:10.1021/acs.jpcc.5b07701