Propagating and banking genetically diverse human sapovirus strains using a human duodenal cell line: investigating antigenic differences between strains

The control of human sapovirus, which causes acute gastroenteritis in individuals of all ages, is challenging because of its association with outbreaks similar to those caused by human norovirus. The establishment of conditions for efficient viral propagation of various viral strains is essential fo...

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Published inJournal of Virology Vol. 98; no. 9; p. e0063924
Main Authors Oka, Tomoichiro, Li, Tian-Cheng, Yonemitsu, Kenzo, Ami, Yasushi, Suzaki, Yuriko, Kataoka, Michiyo, Doan, Yen Hai, Okemoto-Nakamura, Yuko, Kobayashi, Takayuki, Saito, Hiroyuki, Mita, Tetsuo, Tokuoka, Eisuke, Shibata, Shinichiro, Yoshida, Tetsuya, Takagi, Hirotaka
Format Journal Article
LanguageEnglish
Published United States American Society for Microbiology 17.09.2024
Subjects
Animals
Antigens, Viral - genetics
Antigens, Viral - immunology
Bile Acids and Salts
Caliciviridae Infections - immunology
Caliciviridae Infections - virology
Cell Line
Duodenum - immunology
Duodenum - virology
Feces - virology
Gastroenteritis - virology
Genetic Variation
Genotype
Guinea Pigs
Humans
Rabbits
RNA, Viral - genetics
Sapovirus - genetics
Virology
Virus Cultivation
Virus Replication
Virus-Cell Interactions
antigenicity
conventional cell line
sapovirus
norovirus
virus isolation
scalable serial passage
antigen ELISA
conjugated bile acids
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Summary:The control of human sapovirus, which causes acute gastroenteritis in individuals of all ages, is challenging because of its association with outbreaks similar to those caused by human norovirus. The establishment of conditions for efficient viral propagation of various viral strains is essential for understanding the infection mechanism and identifying potential control methods. In this study, two critical factors for human sapovirus propagation in a conventional human duodenal cell line were identified, and 15 strains of different genotypes that differed at the genetic and antigenic levels were isolated and used to prepare virus stocks. The preparation of virus stocks has not been successful for noroviruses, which belong to the same family as sapoviruses. Securing virus stocks of multiple human sapovirus strains represents a significant advance toward establishing a reliable experimental system that does not depend on limited virus-positive fecal material.
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Tomoichiro Oka and Hirotaka Takagi contributed equally to this article.
The authors declare no conflict of interest.
ISSN:0022-538X
1098-5514
1098-5514
DOI:10.1128/jvi.00639-24