Dipropylamine for 9‑Fluorenylmethyloxycarbonyl (Fmoc) Deprotection with Reduced Aspartimide Formation in Solid-Phase Peptide Synthesis

Herein, we report dipropylamine (DPA) as a fluorenylmethyloxycarbonyl (Fmoc) deprotection reagent to strongly reduce aspartimide formation compared to piperidine (PPR) in high-temperature (60 °C) solid-phase peptide synthesis (SPPS). In contrast to PPR, DPA is readily available, inexpensive, low tox...

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Bibliographic Details
Published inACS omega Vol. 8; no. 5; pp. 5050 - 5056
Main Authors Personne, Hippolyte, Siriwardena, Thissa N., Javor, Sacha, Reymond, Jean-Louis
Format Journal Article
LanguageEnglish
Published United States American Chemical Society 07.02.2023
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Summary:Herein, we report dipropylamine (DPA) as a fluorenylmethyloxycarbonyl (Fmoc) deprotection reagent to strongly reduce aspartimide formation compared to piperidine (PPR) in high-temperature (60 °C) solid-phase peptide synthesis (SPPS). In contrast to PPR, DPA is readily available, inexpensive, low toxicity, and nonstench. DPA also provides good yields in SPPS of non-aspartimide-prone peptides and peptide dendrimers.
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ISSN:2470-1343
2470-1343
DOI:10.1021/acsomega.2c07861