Application of an Efficient Gene Targeting System Linking Secondary Metabolites to their Biosynthetic Genes in Aspergillus terreus

Nonribosomal peptides (NRPs) are natural products biosynthesized by NRP synthetases. A kusA-, pyrG- mutant strain of Aspergillus terreus NIH 2624 was developed that greatly facilitated the gene targeting efficiency in this organism. Application of this tool allowed us to link four major types of NRP...

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Published inOrganic letters Vol. 15; no. 14; pp. 3562 - 3565
Main Authors Guo, Chun-Jun, Knox, Benjamin P, Sanchez, James F, Chiang, Yi-Ming, Bruno, Kenneth S, Wang, Clay C. C
Format Journal Article
LanguageEnglish
Published United States American Chemical Society 19.07.2013
Subjects
Aspergillus - chemistry
Aspergillus - genetics
Aspergillus - metabolism
Aspergillus terreus
Biosynthetic Pathways - genetics
chemical reactions
chemical structure
Gene Targeting
genes
ligases
melanin
mutants
organic compounds
Peptide Biosynthesis, Nucleic Acid-Independent - genetics
Peptide Synthases - biosynthesis
Peptide Synthases - chemistry
Peptide Synthases - genetics
Peptide Synthases - metabolism
peptides
secondary metabolites
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Abstract Nonribosomal peptides (NRPs) are natural products biosynthesized by NRP synthetases. A kusA-, pyrG- mutant strain of Aspergillus terreus NIH 2624 was developed that greatly facilitated the gene targeting efficiency in this organism. Application of this tool allowed us to link four major types of NRP-related secondary metabolites to their responsible genes in A. terreus. In addition, an NRP affecting melanin synthesis was also identified in this species.
AbstractList Nonribosomal peptides (NRPs) are natural products biosynthesized by NRP synthetases. A kusA- , pyrG- mutant strain of Aspergillus terreus NIH 2624 was developed that greatly facilitated the gene targeting efficiency in this organism. Application of this tool allowed us to link four major types of NRP related secondary metabolites to their responsible genes in A. terreus . In addition, an NRP affecting melanin synthesis was also identified in this species.
Nonribosomal peptides (NRPs) are natural products biosynthesized by NRP synthetases. A kusA-, pyrG- mutant strain of Aspergillus terreus NIH 2624 was developed that greatly facilitated the gene targeting efficiency in this organism. Application of this tool allowed us to link four major types of NRP-related secondary metabolites to their responsible genes in A. terreus. In addition, an NRP affecting melanin synthesis was also identified in this species.
Nonribosomal peptides (NRPs) are natural products biosynthesized by NRP synthetases. A kusA-, pyrG- mutant strain of Aspergillus terreus NIH 2624 was developed that greatly facilitated the gene targeting efficiency in this organism. Application of this tool allowed us to link four major types of NRP-related secondary metabolites to their responsible genes in A. terreus. In addition, an NRP affecting melanin synthesis was also identified in this species.Nonribosomal peptides (NRPs) are natural products biosynthesized by NRP synthetases. A kusA-, pyrG- mutant strain of Aspergillus terreus NIH 2624 was developed that greatly facilitated the gene targeting efficiency in this organism. Application of this tool allowed us to link four major types of NRP-related secondary metabolites to their responsible genes in A. terreus. In addition, an NRP affecting melanin synthesis was also identified in this species.
Author Wang, Clay C. C
Bruno, Kenneth S
Sanchez, James F
Knox, Benjamin P
Chiang, Yi-Ming
Guo, Chun-Jun
AuthorAffiliation Pacific Northwest National Laboratory
Chia Nan University of Pharmacy and Science
University of Southern California
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– name: University of Southern California
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– name: 2 Chemical and Biological Process Development Group, Energy and Environment Directorate, Pacific Northwest National Laboratory, Richland, WA 99352, USA
– name: 1 Department of Pharmacology and Pharmaceutical Sciences, School of Pharmacy, University of Southern California, Los Angeles, CA 90089, USA
– name: 3 Graduate Institute of Pharmaceutical Science, Chia Nan University of Pharmacy and Science, Tainan 71710, Taiwan
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Snippet Nonribosomal peptides (NRPs) are natural products biosynthesized by NRP synthetases. A kusA-, pyrG- mutant strain of Aspergillus terreus NIH 2624 was developed...
Nonribosomal peptides (NRPs) are natural products biosynthesized by NRP synthetases. A kusA- , pyrG- mutant strain of Aspergillus terreus NIH 2624 was...
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SubjectTerms Aspergillus - chemistry
Aspergillus - genetics
Aspergillus - metabolism
Aspergillus terreus
Biosynthetic Pathways - genetics
chemical reactions
chemical structure
Gene Targeting
genes
ligases
melanin
mutants
organic compounds
Peptide Biosynthesis, Nucleic Acid-Independent - genetics
Peptide Synthases - biosynthesis
Peptide Synthases - chemistry
Peptide Synthases - genetics
Peptide Synthases - metabolism
peptides
secondary metabolites
Title Application of an Efficient Gene Targeting System Linking Secondary Metabolites to their Biosynthetic Genes in Aspergillus terreus
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