Application of an Efficient Gene Targeting System Linking Secondary Metabolites to their Biosynthetic Genes in Aspergillus terreus

Nonribosomal peptides (NRPs) are natural products biosynthesized by NRP synthetases. A kusA-, pyrG- mutant strain of Aspergillus terreus NIH 2624 was developed that greatly facilitated the gene targeting efficiency in this organism. Application of this tool allowed us to link four major types of NRP...

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Bibliographic Details
Published inOrganic letters Vol. 15; no. 14; pp. 3562 - 3565
Main Authors Guo, Chun-Jun, Knox, Benjamin P, Sanchez, James F, Chiang, Yi-Ming, Bruno, Kenneth S, Wang, Clay C. C
Format Journal Article
LanguageEnglish
Published United States American Chemical Society 19.07.2013
Subjects
Aspergillus - chemistry
Aspergillus - genetics
Aspergillus - metabolism
Aspergillus terreus
Biosynthetic Pathways - genetics
chemical reactions
chemical structure
Gene Targeting
genes
ligases
melanin
mutants
organic compounds
Peptide Biosynthesis, Nucleic Acid-Independent - genetics
Peptide Synthases - biosynthesis
Peptide Synthases - chemistry
Peptide Synthases - genetics
Peptide Synthases - metabolism
peptides
secondary metabolites
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Summary:Nonribosomal peptides (NRPs) are natural products biosynthesized by NRP synthetases. A kusA-, pyrG- mutant strain of Aspergillus terreus NIH 2624 was developed that greatly facilitated the gene targeting efficiency in this organism. Application of this tool allowed us to link four major types of NRP-related secondary metabolites to their responsible genes in A. terreus. In addition, an NRP affecting melanin synthesis was also identified in this species.
Bibliography:ObjectType-Article-1
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ISSN:1523-7060
1523-7052
1523-7052
DOI:10.1021/ol401384v