A Nucleoside Derivative 5‑Vinyluridine (VrU) for Imaging RNA in Cells and Animals

In the present study, we used a nucleoside derivative 5-vinyluridine (VrU) for labeling during cell division and for tumor imaging in living mice. We demonstrated that the functional nucleoside bearing a 5-vinyl group is metabolically incorporated into cellular RNA and can be used to image RNA using...

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Published inBioconjugate chemistry Vol. 30; no. 11; pp. 2958 - 2966
Main Authors Liu, Hong-shan, Ishizuka, Takumi, Kawaguchi, Makiko, Nishii, Ryuichi, Kataoka, Hiroaki, Xu, Yan
Format Journal Article
LanguageEnglish
Published United States American Chemical Society 20.11.2019
Subjects
Cell division
Deoxyribonucleic acid
DNA
Fluorescence
Imaging
Mammalian cells
Nucleosides
Reagents
Ribonucleic acid
RNA
Tumors
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Summary:In the present study, we used a nucleoside derivative 5-vinyluridine (VrU) for labeling during cell division and for tumor imaging in living mice. We demonstrated that the functional nucleoside bearing a 5-vinyl group is metabolically incorporated into cellular RNA and can be used to image RNA using a Diels–Alder reaction. The reagent allows for simultaneous and clear imaging of DNA and RNA in mammalian cells at single-cell resolution. We extended this approach to observe DNA and RNA behaviors in several basic stages of cell division. We further demonstrated that the derivative can be used for fluorescence imaging of tumor in live mice.
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ISSN:1043-1802
1520-4812
DOI:10.1021/acs.bioconjchem.9b00643