Characterizing In Situ Methane-Enhanced Biostimulation Potential for 1,4-Dioxane Biodegradation in Groundwater

• Published in: Remediation (New York, N.Y.) Vol. 27; no. 1; pp. 115 - 132
• Main Authors: Sadeghi, Venus, Mora, Rebecca, Jacob, Priya, Chiang, Sheau-Yun (Dora)
• Format: Journal Article
• Language: English
• Published: New York Blackwell Publishing Ltd 01.12.2016; Wiley Subscription Services, Inc
• Subjects: Airport siting; Alkanes; Bacteria; Biodegradation; Groundwater; Methane; Pilots; Remediation; USA, Arizona; USA, Arizona, Tucson
• ISSN: 1051-5658; 1520-6831
• DOI: 10.1002/rem.21500

This study characterizes the 1,4‐dioxane biodegradation potential for an in situ methane‐enhanced biostimulation field pilot study conducted at Air Force Plant 44, located south of the Tucson International Airport in Arizona. In this study, the use of methane as the primary substrate in aerobic cometabolic biodegradation of 1,4‐dioxane is evaluated using environmental molecular diagnostic tools. The findings are compared to an adjacent pilot study, wherein methane was generated via enhanced reductive dechlorination and where methane monooxygenase and methane‐oxidizing bacteria were also found to be abundant. This article also presents the use of 13C and 2H isotopic ratio enrichment, a more recent tool, to support the understanding of 1,4‐dioxane biodegradation in situ. This study is the first of its kind, although alkane gas‐enhanced biodegradation of 1,4‐dioxane has been evaluated extensively in microcosm studies and propane‐enhanced biodegradation of 1,4‐dioxane has been previously studied in the field. ©2016 Wiley Periodicals, Inc.