Printing Patterns of Proteins

Microcontact printing of proteins proves to be an excellent means of directly patterning biomolecules on solid substrates. Monolayer quantities of protein equilibrated on the surface of a hydrophobic, elastomeric stamp are immobilized there to rinses with buffer. These biomolecules can nevertheless...

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Bibliographic Details
Published inLangmuir Vol. 14; no. 9; pp. 2225 - 2229
Main Authors Bernard, André, Delamarche, Emmanuel, Schmid, Heinz, Michel, Bruno, Bosshard, Hans Rudolf, Biebuyck, Hans
Format Journal Article
LanguageEnglish
Published Washington, DC American Chemical Society 28.04.1998
Subjects
Analytical, structural and metabolic biochemistry
Biofilms
Biological and medical sciences
Fundamental and applied biological sciences. Psychology
General aspects, investigation methods
Molecular structure
Monolayers
Proteins
Substrates
Transport properties
Dimethylsiloxane polymer
Biochemical transfer
Atomic force microscopy
Investigation method
Fluorescent labelling
Protein
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Summary:Microcontact printing of proteins proves to be an excellent means of directly patterning biomolecules on solid substrates. Monolayer quantities of protein equilibrated on the surface of a hydrophobic, elastomeric stamp are immobilized there to rinses with buffer. These biomolecules can nevertheless transfer with >99% efficiency from the stamp to a substrate after just 1 s of contact. This capability allows the simple creation of functional patterns of proteins at scales that involve the placement of <1000 molecules in well-defined locations on a surface. The method is suited for the transfer of proteins of many different types onto hydrophilic or hydrophobic substrates.
Bibliography:ark:/67375/TPS-0PBF1BWF-F
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ISSN:0743-7463
1520-5827
DOI:10.1021/la980037l