Postcolumn Infusion as a Quality Control Tool for LC-MS-Based Analysis

Postcolumn infusion has been widely used to study the matrix effect of analytical methods based on liquid chromatography coupled to mass spectrometry (LC-MS). Nevertheless, this methodology is usually only applied during a method development or validation. With this application note, we aim to demon...

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Bibliographic Details
Published inJournal of the American Society for Mass Spectrometry Vol. 33; no. 6; pp. 1077 - 1080
Main Authors González, Oskar, Dubbelman, Anne-Charlotte, Hankemeier, Thomas
Format Journal Article
LanguageEnglish
Published United States American Chemical Society 01.06.2022
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Summary:Postcolumn infusion has been widely used to study the matrix effect of analytical methods based on liquid chromatography coupled to mass spectrometry (LC-MS). Nevertheless, this methodology is usually only applied during a method development or validation. With this application note, we aim to demonstrate that the continuous use of postcolumn infusion can be also a very useful tool to monitor the quality of LC-MS analyses and easily detect flaws in the analytical method performance. Here we propose a protocol that can be transferred to other LC-MS platforms, and we show some real situations in bioanalysis in which postcolumn infusion proved to be extremely helpful in, for example, the evaluation of a sample treatment or the detection of unexpected sources of the matrix effect.
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ISSN:1044-0305
1879-1123
DOI:10.1021/jasms.2c00022