Lithographing of Biomolecules on a Substrate Surface Using an Enzyme-Immobilized AFM Tip

A method of enzymatic lithography was successfully performed in a buffered solution using Staphylococcal serine V8 protease and atomic force microscopy (AFM). The retained activity of the protease immobilized on the AFM tip was confirmed by force measurement to rupture the enzyme−substrate complex....

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Bibliographic Details
Published inNano letters Vol. 3; no. 11; pp. 1471 - 1474
Main Authors Takeda, Seiji, Nakamura, Chikashi, Miyamoto, Chie, Nakamura, Noriyuki, Kageshima, Masami, Tokumoto, Hiroshi, Miyake, Jun
Format Journal Article
LanguageEnglish
Published Washington, DC American Chemical Society 01.11.2003
Subjects
Analytical biochemistry: general aspects, technics, instrumentation
Analytical, structural and metabolic biochemistry
atomic force microscopy
Biological and medical sciences
Fundamental and applied biological sciences. Psychology
lithography
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Summary:A method of enzymatic lithography was successfully performed in a buffered solution using Staphylococcal serine V8 protease and atomic force microscopy (AFM). The retained activity of the protease immobilized on the AFM tip was confirmed by force measurement to rupture the enzyme−substrate complex. After contact scanning using the enzyme-immobilized tip to the substrate peptide layer, a square shape of the lithographed area was clearly observed by subsequent AFM imaging.
Bibliography:ObjectType-Article-2
SourceType-Scholarly Journals-1
ObjectType-Feature-1
content type line 23
ISSN:1530-6984
1530-6992
DOI:10.1021/nl034448k