Structure of Carbon Nanotube Porins in Lipid Bilayers: An in Situ Small-Angle X‑ray Scattering (SAXS) Study

• Published in: Nano letters Vol. 16; no. 7; pp. 4019 - 4024
• Main Authors: Tran, Ich C, Tunuguntla, Ramya H, Kim, Kyunghoon, Lee, Jonathan R. I, Willey, Trevor M, Weiss, Thomas M, Noy, Aleksandr, van Buuren, Tony
• Format: Journal Article
• Language: English
• Published: United States American Chemical Society 13.07.2016
• Subjects: carbon nanotube; INORGANIC, ORGANIC, PHYSICAL AND ANALYTICAL CHEMISTRY; Lipid Bilayers; MATERIALS SCIENCE; Nanopores; NANOSCIENCE AND NANOTECHNOLOGY; Nanotubes, Carbon; Phospholipid; porins; Scattering, Small Angle; small-angle X-ray scattering (SAXS); X-Ray Diffraction; Phospholipid; carbon nanotube; porins; small-angle X-ray scattering (SAXS)
• ISSN: 1530-6984; 1530-6992
• DOI: 10.1021/acs.nanolett.6b00466

Carbon nanotube porins (CNTPs), small segments of carbon nanotubes capable of forming defined pores in lipid membranes, are important future components for bionanoelectronic devices as they could provide a robust analog of biological membrane channels. In order to control the incorporation of these CNT channels into lipid bilayers, it is important to understand the structure of the CNTPs before and after insertion into the lipid bilayer as well as the impact of such insertion on the bilayer structure. Here we employed a noninvasive in situ probe, small-angle X-ray scattering, to study the integration of CNT porins into dioleoylphosphatidylcholine bilayers. Our results show that CNTPs in solution are stabilized by a monolayer of lipid molecules wrapped around their outer surface. We also demonstrate that insertion of CNTPs into the lipid bilayer results in decreased bilayer thickness with the magnitude of this effect increasing with the concentration of CNTPs.