Investigating the Transport Dynamics of Anthocyanins from Unprocessed Fruit and Processed Fruit Juice from Sour Cherry (Prunus cerasus L.) across Intestinal Epithelial Cells

• Published in: Journal of agricultural and food chemistry Vol. 61; no. 47; pp. 11434 - 11441
• Main Authors: Toydemir, Gamze, Boyacioglu, Dilek, Capanoglu, Esra, van der Meer, Ingrid M, Tomassen, Monic M. M, Hall, Robert D, Mes, Jurriaan J, Beekwilder, Jules
• Format: Journal Article
• Language: English
• Published: Washington, DC American Chemical Society 27.11.2013
• Subjects: absorption; anthocyanins; Anthocyanins - pharmacokinetics; antioxidants; Beverages; bioavailability; Biological and medical sciences; Biological Availability; caco-2; Caco-2 Cells; cellular uptake; cherries; citric acid; consumption; Culture Media - chemistry; Culture Media - pharmacology; dietary; epithelial cells; Epithelial Cells - drug effects; Food Handling; food matrix; Fruit - chemistry; fruit juices; Fundamental and applied biological sciences. Psychology; glucosides; human cell lines; human health; Humans; intestinal absorption; intestinal mucosa; Intestines - cytology; Intestines - drug effects; Intestines - metabolism; polyphenols; processed foods; protein interactions; Prunus - chemistry; Prunus cerasus; quercetin; red grape juice; Solid Phase Extraction; sucrose; Prunus cerasus L; sour cherry; anthocyanins; bioavailability; in vitro; Caco-2 cell model; nectar processing; Anthocyanin; Fruit; Rosaceae; Gut; Bioavailability; In vitro; Fruit juice; Prunus cerasus; Dynamics; Dicotyledones; Angiospermae; Epithelial cell; Spermatophyta; Models; Operating process; Transport
• ISSN: 0021-8561; 1520-5118
• DOI: 10.1021/jf4032519

Anthocyanins can contribute to human health through preventing a variety of diseases. The uptake of these compounds from food and the parameters determining uptake efficiency within the human body are still poorly understood. Here we have employed a Caco-2 cell based system to investigate the transport of key antioxidant food components from sour cherry (Prunus cerasus L.) across the intestinal epithelial barrier. Anthocyanins and (−)-epicatechin were supplied in three contrasting matrices: fruit, processed fruit cherry juice, and polyphenolic fractions obtained by solid-phase extraction. Results show that both compound types behave differently. Fruit or juice matrices display comparable transport across the epithelial cell layer. The juice supplements sucrose and citric acid, which are regularly added to processed foods, have a positive effect on stability and transport. Polyphenolic fractions display a lower transport efficiency, relative to that of the fruit or juice, indicating the importance of food matrix components for intestinal absorption of polyphenols.