Toroidal Packaging of pDNA into Block Ionomer Micelles Exerting Promoted in Vivo Gene Expression

• Published in: Biomacromolecules Vol. 16; no. 9; pp. 2664 - 2671
• Main Authors: Li, Yanmin, Osada, Kensuke, Chen, Qixian, Tockary, Theofilus A, Dirisala, Anjaneyulu, Takeda, Kaori M, Uchida, Satoshi, Nagata, Kazuya, Itaka, Keiji, Kataoka, Kazunori
• Format: Journal Article
• Language: English
• Published: United States American Chemical Society 14.09.2015
• Subjects: Animals; Gene Expression; Mice; Micelles; Muscle, Skeletal - metabolism; Plasmids - chemistry; Plasmids - pharmacology; Transcription, Genetic; Transduction, Genetic - methods
• ISSN: 1525-7797; 1526-4602
• DOI: 10.1021/acs.biomac.5b00491

Selectively spooling single plasmid DNA (pDNA), as a giant polyelectrolyte, into a nanosized toroidal structure or folding it into a rod-like structure has been accomplished by polyion complexation with block catiomers to form polymeric micelles in varying NaCl concentrations. The interactive potency between the pDNA and block catiomers was determined to play a critical role in defining the ultimate structure of the pDNA; the formation of toroidal or rod-like structures was achieved by complexation in 600 or 0 mM NaCl solutions, respectively. Compared with the rod-like structure, the toroidal structure possessed superior biological functions capable not only of elevating in vitro transcription but also of elevating in vivo gene transduction efficiency. This demonstrated the great utility of the toroidal pDNA packaging as a distinct structured gene carrier. Furthermore, the fact that the NaCl concentration at which the toroidal structure was specifically formed corresponds to seawater stimulates interest in this ordered nanostructure as a possible inherent structure for DNA.