Anti-Biofilm Enzymes-Assisted Antibiotic Therapy against Burn Wound Infection by Pseudomonas aeruginosa

Pseudomonas aeruginosa can form biofilms at the site of burn wound, leading to infection and the failure of treatment regimens. The previous study demonstrated that a combination of the quorum-quenching enzyme AidH and the extracellular matrix hydrolase PslG was effective in inhibiting biofilm and p...

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Published inAntimicrobial agents and chemotherapy Vol. 67; no. 7; p. e0030723
Main Authors Zhang, Yixin, Liu, Xiaolong, Wen, Huamei, Cheng, Zhongle, Zhang, Yanyu, Zhang, Haichuan, Mi, Zhongwen, Fan, Xinjiong
Format Journal Article
LanguageEnglish
Published United States American Society for Microbiology 18.07.2023
Subjects
Anti-Bacterial Agents - pharmacology
Anti-Bacterial Agents - therapeutic use
Antimicrobial Chemotherapy
Biofilms
Burns - complications
Burns - drug therapy
Burns - microbiology
Communicable Diseases
Experimental Therapeutics
Humans
Pseudomonas aeruginosa
Pseudomonas Infections - drug therapy
Pseudomonas Infections - microbiology
Tobramycin - pharmacology
Tobramycin - therapeutic use
Wound Infection - microbiology
Pseudomonas aeruginosa
combination therapy
anti-biofilm enzyme
burn wound infection
tobramycin
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Summary:Pseudomonas aeruginosa can form biofilms at the site of burn wound, leading to infection and the failure of treatment regimens. The previous study demonstrated that a combination of the quorum-quenching enzyme AidH and the extracellular matrix hydrolase PslG was effective in inhibiting biofilm and promoting antibiotic synergy. The aim of the present study was to evaluate the efficacy of this combination of enzymes in conjunction with tobramycin in treating burn wound infected with P. aeruginosa. The results showed that this treatment was effective in quorum-quenching and biofilm inhibition on infected wounds. Compared with the tobramycin treatment only, simultaneous treatment with the enzymes and antibiotics significantly reduced the severity of tissue damage, decreased the bacterial load, and reduced the expression of the inflammatory indicators myeloperoxidase (MPO) and malondialdehyde (MDA). Topical application of the enzymes also reduced the bacterial load and inflammation to some extent. These results indicate that the combined-enzyme approach is a potentially effective treatment for P. aeruginosa biofilm infections of burn wounds.
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The authors declare no conflict of interest.
Yixin Zhang and Xiaolong Liu have an equal contribution. Author order was determined by drawing straws.
ISSN:0066-4804
1098-6596
DOI:10.1128/aac.00307-23