Real-Time PCR Targeting the penA Mosaic XXXIV Type for Prediction of Extended-Spectrum-Cephalosporin Susceptibility in Clinical Neisseria gonorrhoeae Isolates

isolates with decreased susceptibility to extended-spectrum cephalosporins (ESCs) are increasing. We developed an assay to predict susceptibility to ESCs by targeting mosaic XXXIV, an allele prevalent among U.S. isolates with elevated ESC MICs. The assay was 97% sensitive and 100% specific for predi...

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Published inAntimicrobial agents and chemotherapy Vol. 61; no. 11
Main Authors Wong, L K, Hemarajata, P, Soge, O O, Humphries, R M, Klausner, J D
Format Journal Article
LanguageEnglish
Published United States American Society for Microbiology 01.11.2017
Subjects
Bacterial Proteins
Cephalosporins
Epidemiology and Surveillance
Neisseria gonorrhoeae
Real-Time Polymerase Chain Reaction
extended-spectrum cephalosporins
molecular methods
ceftriaxone
Neisseria gonorrhoeae
antibiotic resistance
antimicrobial resistance
gonorrhea
cephalosporin
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Summary:isolates with decreased susceptibility to extended-spectrum cephalosporins (ESCs) are increasing. We developed an assay to predict susceptibility to ESCs by targeting mosaic XXXIV, an allele prevalent among U.S. isolates with elevated ESC MICs. The assay was 97% sensitive and 100% specific for predicting at least one ESC MIC above the CDC alert value among clinical isolates, and it could be multiplexed with a previously validated PCR to predict ciprofloxacin susceptibility.
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Citation Wong LK, Hemarajata P, Soge OO, Humphries RM, Klausner JD. 2017. Real-time PCR targeting the penA mosaic XXXIV type for prediction of extended-spectrum-cephalosporin susceptibility in clinical Neisseria gonorrhoeae isolates. Antimicrob Agents Chemother 61:e01339-17. https://doi.org/10.1128/AAC.01339-17.
ISSN:0066-4804
1098-6596
DOI:10.1128/AAC.01339-17