Limit of Detection for Rapid Antigen Testing of the SARS-CoV-2 Omicron and Delta Variants of Concern Using Live-Virus Culture

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Bibliographic Details
Published inJournal of clinical microbiology Vol. 60; no. 5; p. e0014022
Main Authors Stanley, Sydney, Hamel, Donald J, Wolf, Ian D, Riedel, Stefan, Dutta, Sanjucta, Contreras, Elisa, Callahan, Cody J, Cheng, Annie, Arnaout, Ramy, Kirby, James E, Kanki, Phyllis J
Format Journal Article
LanguageEnglish
Published United States American Society for Microbiology 18.05.2022
Subjects
Clinical Microbiology
COVID-19 - diagnosis
Humans
Immunologic Tests
Letter to the Editor
Limit of Detection
SARS-CoV-2 - genetics
COVID-19
SARS-CoV-2
Delta
lateral flow assay
Omicron
analytical sensitivity
limit of detection
RT-qPCR
antigen test
Online AccessGet full text

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Author Wolf, Ian D
Hamel, Donald J
Callahan, Cody J
Cheng, Annie
Arnaout, Ramy
Stanley, Sydney
Contreras, Elisa
Kirby, James E
Riedel, Stefan
Kanki, Phyllis J
Dutta, Sanjucta
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  givenname: Sanjucta
  surname: Dutta
  fullname: Dutta, Sanjucta
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  surname: Cheng
  fullname: Cheng, Annie
  organization: Department of Pathology, Beth Israel Deaconess Medical Centergrid.239395.7, Boston, Massachusetts, USA
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  organization: Department of Immunology and Infectious Diseases, Harvard T. H. Chan School of Public Health, Boston, Massachusetts, USA
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Issue 5
Keywords COVID-19
SARS-CoV-2
Delta
lateral flow assay
Omicron
analytical sensitivity
limit of detection
RT-qPCR
antigen test
Language English
License All Rights Reserved. This article is made available via the PMC Open Access Subset for unrestricted noncommercial re-use and secondary analysis in any form or by any means with acknowledgement of the original source. These permissions are granted for the duration of the World Health Organization (WHO) declaration of COVID-19 as a global pandemic. https://doi.org/10.1128/ASMCopyrightv2
All Rights Reserved.
This article is made available via the PMC Open Access Subset for unrestricted noncommercial re-use and secondary analysis in any form or by any means with acknowledgement of the original source. These permissions are granted for the duration of the World Health Organization (WHO) declaration of COVID-19 as a global pandemic.
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Elisa Contreras and Cody J. Callahan contributed equally to this article. Author order was randomly assigned by the corresponding authors. James E. Kirby and Phyllis J. Kanki are co-senior authors.
The authors declare a conflict of interest. LumiraDx provided instrumentation and diagnostic kits; Abbott provided diagnostic Ag kits; and Ginkgo Bioworks provided CareStart and Genbody antigen testing kits for the study. The BIDMC authors (J.E.K., A.C.) received support from Abbott Molecular unrelated to this study under a COVID-19 Diagnostics Evaluation Agreement. Abbott, LumiraDx, and Ginkgo Bioworks had no role in study design, manuscript preparation or decision to publish. All authors, no other conflicts of interest.
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SubjectTerms Clinical Microbiology
COVID-19 - diagnosis
Humans
Immunologic Tests
Letter to the Editor
Limit of Detection
SARS-CoV-2 - genetics
Title Limit of Detection for Rapid Antigen Testing of the SARS-CoV-2 Omicron and Delta Variants of Concern Using Live-Virus Culture
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