Inhibition of aminoglycoside 6'-N-acetyltransferase type Ib-mediated amikacin resistance in Klebsiella pneumoniae by zinc and copper pyrithione

The in vitro activity of the aminoglycoside 6'-N-acetyltransferase type Ib [AAC(6')-Ib] was inhibited by CuCl2 with a 50% inhibitory concentration (IC50) of 2.8 μM. The growth of an amikacin-resistant Klebsiella pneumoniae strain isolated from a neonate with meningitis was inhibited when a...

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Published inAntimicrobial agents and chemotherapy Vol. 59; no. 9; pp. 5851 - 5853
Main Authors Chiem, Kevin, Fuentes, Brooke A, Lin, David L, Tran, Tung, Jackson, Alexis, Ramirez, Maria S, Tolmasky, Marcelo E
Format Journal Article
LanguageEnglish
Published United States American Society for Microbiology 01.09.2015
Subjects
Acetyltransferases
Amikacin
Amikacin - pharmacology
Aminoglycosides - pharmacology
Anti-Bacterial Agents - pharmacology
Experimental Therapeutics
Klebsiella pneumoniae
Klebsiella pneumoniae - drug effects
Klebsiella pneumoniae - enzymology
Klebsiella pneumoniae - metabolism
Microbial Sensitivity Tests
Organometallic Compounds
Organometallic Compounds - pharmacology
Pyridines
Pyridines - pharmacology
Zinc
Zinc - pharmacology
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Summary:The in vitro activity of the aminoglycoside 6'-N-acetyltransferase type Ib [AAC(6')-Ib] was inhibited by CuCl2 with a 50% inhibitory concentration (IC50) of 2.8 μM. The growth of an amikacin-resistant Klebsiella pneumoniae strain isolated from a neonate with meningitis was inhibited when amikacin was supplemented by the addition of Zn(2+) or Cu(2+) in complex with the ionophore pyrithione. Coordination complexes between cations and ionophores could be developed for their use, in combination with aminoglycosides, to treat resistant infections.
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Citation Chiem K, Fuentes BA, Lin DL, Tran T, Jackson A, Ramirez MS, Tolmasky ME. 2015. Inhibition of aminoglycoside 6′-N-acetyltransferase type Ib-mediated amikacin resistance in Klebsiella pneumoniae by zinc and copper pyrithione. Antimicrob Agents Chemother 59:5851–5853. doi:10.1128/AAC.01106-15.
ISSN:0066-4804
1098-6596
DOI:10.1128/AAC.01106-15