Inhibition of the Classical Pathway of Complement Activation Impairs Bacterial Clearance during Enterococcus faecalis Infection

• Published in: Infection and immunity Vol. 89; no. 5
• Main Authors: Shehab El-Din, Eman M Rabie, Elgaml, Abdelaziz, Ali, Youssif M, Hassan, Ramadan
• Format: Journal Article
• Language: English
• Published: United States American Society for Microbiology 16.04.2021
• Subjects: Bacterial Infections; Enterococcus faecalis; classical pathway; C1s; innate immunity; Fab fragments; complement system
• ISSN: 0019-9567; 1098-5522
• DOI: 10.1128/IAI.00660-20

infections are considered a major public health concern worldwide. The complement system has a crucial role in the protection against different microbial pathogens, including Complement can be activated through three different pathways, including the classical, lectin, and alternative pathways. There is limited information on the role of the classical pathway (CP) in protection against infections caused by In the present study, we generated Fab fragments that successfully block the CP in mouse via inhibition of a key enzyme, C1s-A. Our results showed that anti-C1s-A Fab fragments block CP-mediated C3b and C4b deposition We further showed that administration of anti-C1s-A Fab fragments significantly impairs the CP functional activity Moreover, treatment of mice infected with using anti-C1s-A Fab fragments significantly impairs bacterial clearance as determined from the viable bacterial counts recovered from blood, kidneys, spleens, livers, and lungs of infected mice. Overall, this study highlights the essential role of the CP in host defense against .