Retained Activity of an O25b-Specific Monoclonal Antibody against an Mcr-1-Producing Escherichia coli Sequence Type 131 Strain

Plasmid-encoded colistin resistance is emerging among extraintestinal pathogenic strains, including those of the epidemic clone sequence type 131 (ST131)-H30. Mcr-1 transfers a phosphoethanolamine to the lipid A portion of lipopolysaccharide (LPS), conferring resistance to polymyxins. We investigate...

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Published inAntimicrobial agents and chemotherapy Vol. 62; no. 7
Main Authors Guachalla, Luis M, Ramoni, Katharina, Varga, Cecilia, Mutti, Michele, Ghazawi, Akela, Pál, Tibor, Nagy, Eszter, Sonnevend, Ágnes, Nagy, Gábor, Szijártó, Valéria
Format Journal Article
LanguageEnglish
Published United States American Society for Microbiology 01.07.2018
Subjects
Anti-Bacterial Agents
Antibodies, Monoclonal
Escherichia coli Infections
Escherichia coli Proteins
Experimental Therapeutics
Extraintestinal Pathogenic Escherichia coli
Escherichia coli ST131
mcr-1
monoclonal antibodies
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Summary:Plasmid-encoded colistin resistance is emerging among extraintestinal pathogenic strains, including those of the epidemic clone sequence type 131 (ST131)-H30. Mcr-1 transfers a phosphoethanolamine to the lipid A portion of lipopolysaccharide (LPS), conferring resistance to polymyxins. We investigated whether this modification changed the activity of the monoclonal antibody ASN-4, specific to the O25b side chain of ST131 LPS. We confirmed that, unlike colistin, ASN-4 retained its bactericidal and endotoxin-neutralizing activities and therefore offers a treatment option against extremely drug-resistant ST131 isolates.
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Citation Guachalla LM, Ramoni K, Varga C, Mutti M, Ghazawi A, Pál T, Nagy E, Sonnevend Á, Nagy G, Szijártó V. 2018. Retained activity of an O25b-specific monoclonal antibody against an Mcr-1-producing Escherichia coli sequence type 131 strain. Antimicrob Agents Chemother 62:e00046-18. https://doi.org/10.1128/AAC.00046-18.
ISSN:0066-4804
1098-6596
DOI:10.1128/AAC.00046-18