Dynamical and Phase Behavior of a Phospholipid Membrane Altered by an Antimicrobial Peptide at Low Concentration

• Published in: The journal of physical chemistry letters Vol. 7; no. 13; pp. 2394 - 2401
• Main Authors: Sharma, V. K, Mamontov, E, Tyagi, M, Qian, S, Rai, D. K, Urban, V. S
• Format: Journal Article
• Language: English
• Published: United States American Chemical Society 07.07.2016
• Subjects: alamethicin; antimicrobial peptides; BASIC BIOLOGICAL SCIENCES; dynamics; melittin; membrane-peptide interactions; neutron scattering; phosphocholine membrane
• ISSN: 1948-7185; 1948-7185
• DOI: 10.1021/acs.jpclett.6b01006

The mechanism of action of antimicrobial peptides is traditionally attributed to the formation of pores in the lipid cell membranes of pathogens, which requires a substantial peptide to lipid ratio. However, using incoherent neutron scattering, we show that even at a concentration too low for pore formation, an archetypal antimicrobial peptide, melittin, disrupts the regular phase behavior of the microscopic dynamics in a phospholipid membrane, dimyristoylphosphatidylcholine (DMPC). At the same time, another antimicrobial peptide, alamethicin, does not exert a similar effect on the DMPC microscopic dynamics. The melittin-altered lateral motion of DMPC at physiological temperature no longer resembles the fluid-phase behavior characteristic of functional membranes of the living cells. The disruptive effect demonstrated by melittin even at low concentrations reveals a new mechanism of antimicrobial action relevant in more realistic scenarios, when peptide concentration is not as high as would be required for pore formation, which may facilitate treatment with antimicrobial peptides.