Nanoscale Metal–Organic Frameworks for Ratiometric Oxygen Sensing in Live Cells

We report the design of a phosphorescence/fluorescence dual-emissive nanoscale metal–organic framework (NMOF), R-UiO, as an intracellular oxygen (O2) sensor. R-UiO contains a Pt­(II)-porphyrin ligand as an O2-sensitive probe and a Rhodamine-B isothiocyanate ligand as an O2-insensitive reference prob...

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Published inJournal of the American Chemical Society Vol. 138; no. 7; pp. 2158 - 2161
Main Authors Xu, Ruoyu, Wang, Youfu, Duan, Xiaopin, Lu, Kuangda, Micheroni, Daniel, Hu, Aiguo, Lin, Wenbin
Format Journal Article
LanguageEnglish
Published United States American Chemical Society 24.02.2016
Subjects
Animals
Biocompatible Materials - chemical synthesis
Biocompatible Materials - chemistry
Biosensing Techniques
Cell Line, Tumor
Cell Survival
Metalloporphyrins - chemical synthesis
Metalloporphyrins - chemistry
Mice
Nanostructures - chemistry
Oxygen - analysis
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Summary:We report the design of a phosphorescence/fluorescence dual-emissive nanoscale metal–organic framework (NMOF), R-UiO, as an intracellular oxygen (O2) sensor. R-UiO contains a Pt­(II)-porphyrin ligand as an O2-sensitive probe and a Rhodamine-B isothiocyanate ligand as an O2-insensitive reference probe. It exhibits good crystallinity, high stability, and excellent ratiometric luminescence response to O2 partial pressure. In vitro experiments confirmed the applicability of R-UiO as an intracellular O2 biosensor. This work is the first report of a NMOF-based intracellular oxygen sensor and should inspire the design of ratiometric NMOF sensors for other important analytes in biological systems.
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R.X. and Y.W. contributed equally.
ISSN:0002-7863
1520-5126
DOI:10.1021/jacs.5b13458