Identification and Characterization of the Chaetoviridin and Chaetomugilin Gene Cluster in Chaetomium globosum Reveal Dual Functions of an Iterative Highly-Reducing Polyketide Synthase

We report the identification and characterization of the caz biosynthetic cluster from Chaetomium globosum and the characterization of a highly reducing polyketide synthase (PKS) that acts in both a sequential and convergent manner with a nonreducing PKS to form the chaetomugilin and chaetoviridin a...

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Bibliographic Details
Published inJournal of the American Chemical Society Vol. 134; no. 43; pp. 17900 - 17903
Main Authors Winter, Jaclyn M, Sato, Michio, Sugimoto, Satoru, Chiou, Grace, Garg, Neil K, Tang, Yi, Watanabe, Kenji
Format Journal Article
LanguageEnglish
Published United States American Chemical Society 31.10.2012
Subjects
Chaetomium - genetics
Furans - chemistry
Molecular Structure
Polyketide Synthases - antagonists & inhibitors
Polyketide Synthases - metabolism
Pyrones - chemistry
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Summary:We report the identification and characterization of the caz biosynthetic cluster from Chaetomium globosum and the characterization of a highly reducing polyketide synthase (PKS) that acts in both a sequential and convergent manner with a nonreducing PKS to form the chaetomugilin and chaetoviridin azaphilones. Genetic inactivation studies verified the involvement of individual caz genes in the biosynthesis of the azaphilones. Through in vitro reconstitution, we demonstrated the in vitro synthesis of chaetoviridin A from the pyranoquinone intermediate cazisochromene using the highly reducing PKS and an acyltransferase.
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ISSN:0002-7863
1520-5126
DOI:10.1021/ja3090498