Perfluorophenyl Azide Immobilization Chemistry for Single Molecule Force Spectroscopy of the Concanavalin A/Mannose Interaction

• Published in: Langmuir Vol. 26; no. 22; pp. 16677 - 16680
• Main Authors: Madwar, Carolin, Chu Kwan, William, Deng, Lingquan, Ramström, Olof, Schmidt, Rolf, Zou, Shan, Cuccia, Louis A
• Format: Journal Article
• Language: English
• Published: Washington, DC American Chemical Society 16.11.2010
• Subjects: adhesion; Azides - chemistry; bond; cells; Chemistry; Concanavalin A - metabolism; Exact sciences and technology; General and physical chemistry; Hydrocarbons, Fluorinated - chemistry; Kemi; Mannose - chemistry; Mannose - metabolism; microscopy; Microscopy, Atomic Force; NATURAL SCIENCES; NATURVETENSKAP; Organic chemistry; Organisk kemi; Photochemical Processes; Protein Binding; Surface Properties; Gold; Force; Glass; Immobilization; Dissociation constant; Polymer; Transition metal; Mannose; Azides; Chemistry; Dynamics; Chemical properties; Rate constant
• ISSN: 0743-7463; 1520-5827; 1520-5827
• DOI: 10.1021/la1036579

The versatility of perfluorophenyl azide (PFPA) derivatives makes them useful for attaching a wide variety of biomolecules and polymers to surfaces. Herein, a single molecule force spectroscopy (SMFS) study of the concanavalin A/mannose interaction was carried out using PFPA immobilization chemistry. SMFS of the concanavalin A/mannose interaction yielded an average unbinding force of 70−80 pN for loading rates between 8000 and 40 000 pN/s for mannose surfaces on aminated glass, and an unbinding force of 57 ± 20 pN at 6960 pN/s for mannose surfaces on gold-coated glass. Dynamic force spectroscopy was used to determine the dissociation rate constant, k off, for this interaction to be 0.16 s−1.