In Vivo Biodistribution and Clearance Studies Using Multimodal Organically Modified Silica Nanoparticles

• Published in: ACS nano Vol. 4; no. 2; pp. 699 - 708
• Main Authors: Kumar, Rajiv, Roy, Indrajit, Ohulchanskky, Tymish Y, Vathy, Lisa A, Bergey, Earl J, Sajjad, Munawwar, Prasad, Paras N
• Format: Journal Article
• Language: English
• Published: United States American Chemical Society 23.02.2010
• Subjects: Animals; Biliary Tract - metabolism; Drug-Related Side Effects and Adverse Reactions; Female; Fluorescent Dyes - chemistry; Gamma Rays; Injections; Iodine Radioisotopes - chemistry; Light; Liver - metabolism; Metabolic Clearance Rate; Mice; Microscopy; Molecular Imaging; Nanoparticles - chemistry; Organometallic Compounds - administration & dosage; Organometallic Compounds - chemistry; Organometallic Compounds - pharmacokinetics; Organometallic Compounds - toxicity; Silicon Dioxide - chemistry; 124I radiolabeling; NIR fluorophore; biodistribution; clearance and toxicity; ORMOSIL nanoparticles; optical and PET imaging
• ISSN: 1936-0851; 1936-086X
• DOI: 10.1021/nn901146y

Successful translation of the use of nanoparticles from laboratories to clinics requires exhaustive and elaborate studies involving the biodistribution, clearance, and biocompatibility of nanoparticles for in vivo biomedical applications. We report here the use of multimodal organically modified silica (ORMOSIL) nanoparticles for in vivo bioimaging, biodistribution, clearance, and toxicity studies. We have synthesized ORMOSIL nanoparticles with diameters of 20−25 nm, conjugated with near-infrared (NIR) fluorophores and radiolabeled them with 124I, for optical and PET imaging in vivo. The biodistribution of the nontargeted nanoparticles was studied in nontumored nude mice by optical fluorescence imaging, as well by measuring the radioactivity from harvested organs. Biodistribution studies showed a greater accumulation of nanoparticles in liver, spleen, and stomach than in kidney, heart, and lungs. The clearance studies carried out over a period of 15 days indicated hepatobiliary excretion of the nanoparticles. Selected tissues were analyzed for any potential toxicity by histological analysis, which confirmed the absence of any adverse effect or any other abnormalities in the tissues. The results demonstrate that these multimodal nanoparticles have potentially ideal attributes for use as biocompatible probes for in vivo imaging.