Lactobacillus acidophilus protects against Corynebacterium pseudotuberculosis infection by regulating the autophagy of macrophages and maintaining gut microbiota homeostasis in C57BL/6 mice

( ), a facultative intracellular bacterium, is an important zoonotic pathogen that causes abscesses and pyogenic granulomas. The relationship between gut microbiota and host health or diseases has received increasing attention. However, the role of gut microbiota in the process of infection is still...

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Published inmSystems Vol. 9; no. 7; p. e0048424
Main Authors Li, Dengliang, Jiang, Yuecai, Cui, Zhanding, Ma, Mengzhen, Zhu, Fang, Li, Guanhua, Yang, Haoyue, Li, Shaofei, Zhang, Tianliang, Chen, Dekun, Ma, Wentao
Format Journal Article
LanguageEnglish
Published United States American Society for Microbiology 23.07.2024
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Summary:( ), a facultative intracellular bacterium, is an important zoonotic pathogen that causes abscesses and pyogenic granulomas. The relationship between gut microbiota and host health or diseases has received increasing attention. However, the role of gut microbiota in the process of infection is still unclear. In this study, we established a infection model in C57BL/6 mice and examined the impact of preemptive oral administration ( ) on infection. Our findings revealed that infection led to pronounced pathological alterations in the liver and kidneys, characterized by abscess formation, intense inflammatory responses, and bacterial overload. Remarkably, these deleterious effects were greatly relieved by oral administration of before infection with . Additionally, we further found that during infection, peritoneal macrophages (PMs) of mice orally administered with accumulated more rapidly at sites of infection. Furthermore, our results showed that PMs from mice with oral administration showed a stronger clearance effect, and this was mediated by high expression of LC3-II protein. Meanwhile, oral administration of protected the gut microbiota disorder in C57BL/6 mice caused by infection. In summary, our study demonstrates that oral administration of confers effective protection against infection in C57BL/6 mice by modulating macrophage autophagy, thereby augmenting bacterial clearance and preserving gut microbiota and function stability. These findings position as a viable probiotic candidate for the clinical prevention of infection. (C. ) is known to induce a range of chronic diseases in both animals and humans. Currently, clinical treatment for C. infection mainly relies on antibiotic therapy or surgical intervention. However, excessive use of antibiotics may increase the risk of drug-resistant strains, and the effectiveness of treatment remains unsatisfactory. Furthermore, surgical procedures do not completely eradicate pathogens and can easily cause environmental pollution. Probiotic interventions are receiving increasing attention for improving the body's immune system and maintaining health. In this study, we established a C. infection model in C57BL/6 mice to explore the impact of acidophilus during C. infection. Our results showed that effectively protected against C. infection by regulating the autophagy of macrophages and maintaining intestinal microbiota homeostasis. This study may provide a new strategy for the prevention of C. infection.
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The authors declare no conflict of interest.
ISSN:2379-5077
2379-5077
DOI:10.1128/msystems.00484-24