PEG Thiazolidine-2-thione, a Novel Reagent for Facile Protein Modification:  Conjugation of Bovine Hemoglobin

A novel PEG linker that employs a thiazolidine-2-thione group has been synthesized. Kinetic studies done on this compound demonstrate a relatively long half-life compared to those of traditional succinimidyl linkers. This new PEG derivative reacts with proteins under mild conditions and was utilized...

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Published inBioconjugate chemistry Vol. 7; no. 6; pp. 638 - 641
Main Authors Greenwald, Richard B, Pendri, Annapurna, Martinez, Anthony, Gilbert, Carl, Bradley, Patricia
Format Journal Article
LanguageEnglish
Published United States American Chemical Society 01.11.1996
Subjects
Animals
Cattle
Chromatography, High Pressure Liquid
Hemoglobins - chemistry
Indicators and Reagents
Polyethylene Glycols
Thiazoles - chemistry
Thiazolidines
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Summary:A novel PEG linker that employs a thiazolidine-2-thione group has been synthesized. Kinetic studies done on this compound demonstrate a relatively long half-life compared to those of traditional succinimidyl linkers. This new PEG derivative reacts with proteins under mild conditions and was utilized to conjugate bovine hemoglobin (bHb) to provide a PEG amide-linked protein. The physical characteristics of this conjugate were compared with those of the known PEG carbamate-linked bHb.
Bibliography:Abstract published in Advance ACS Abstracts, October 15, 1996.
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ISSN:1043-1802
1520-4812
DOI:10.1021/bc960059o