Switchable Fluorescent Imaging of Intracellular Telomerase Activity Using Telomerase-Responsive Mesoporous Silica Nanoparticle

• Published in: Journal of the American Chemical Society Vol. 135; no. 36; pp. 13282 - 13285
• Main Authors: Qian, Ruocan, Ding, Lin, Ju, Huangxian
• Format: Journal Article
• Language: English
• Published: United States American Chemical Society 11.09.2013
• Subjects: DNA; DNA - chemistry; drugs; fluorescein; Fluorescence; Fluorescent Dyes - chemistry; HeLa Cells; Humans; image analysis; monitoring; nanoparticles; Nanoparticles - chemistry; Particle Size; Porosity; porous media; silica; Silicon Dioxide - chemistry; standard operating procedures; Surface Properties; telomerase; Telomerase - chemistry; Telomerase - metabolism

This work designs a telomerase-responsive mesoporous silica nanoparticle (MSN) to realize in situ “off-on” imaging of intracellular telomerase activity. In the wrapping DNA (O1) sealed MSN probe, a black hole fluorescence quencher is covalently immobilized on the inner walls of the mesopores, while...