Switchable Fluorescent Imaging of Intracellular Telomerase Activity Using Telomerase-Responsive Mesoporous Silica Nanoparticle

This work designs a telomerase-responsive mesoporous silica nanoparticle (MSN) to realize in situ “off-on” imaging of intracellular telomerase activity. In the wrapping DNA (O1) sealed MSN probe, a black hole fluorescence quencher is covalently immobilized on the inner walls of the mesopores, while...

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Bibliographic Details
Published inJournal of the American Chemical Society Vol. 135; no. 36; pp. 13282 - 13285
Main Authors Qian, Ruocan, Ding, Lin, Ju, Huangxian
Format Journal Article
LanguageEnglish
Published United States American Chemical Society 11.09.2013
Subjects
DNA
DNA - chemistry
drugs
fluorescein
Fluorescence
Fluorescent Dyes - chemistry
HeLa Cells
Humans
image analysis
monitoring
nanoparticles
Nanoparticles - chemistry
Particle Size
Porosity
porous media
silica
Silicon Dioxide - chemistry
standard operating procedures
Surface Properties
telomerase
Telomerase - chemistry
Telomerase - metabolism
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Summary:This work designs a telomerase-responsive mesoporous silica nanoparticle (MSN) to realize in situ “off-on” imaging of intracellular telomerase activity. In the wrapping DNA (O1) sealed MSN probe, a black hole fluorescence quencher is covalently immobilized on the inner walls of the mesopores, while fluorescein is loaded in the mesopores. In the presence of telomerase and dNTPs, the designed O1 can be extended and then moves away from the MSN surface via forming a rigid hairpin-like DNA structure. Thus the O1 can act as a “biogate” to block and release fluorescein for “off-on” switchable fluorescent imaging. The MSN probe exhibits good performance for sensitive in situ tracking of telomerase activity in living cells. The practicality of this protocol has been verified by monitoring the change of cellular telomerase activity in response to telomerase-related drugs.
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ISSN:0002-7863
1520-5126
1520-5126
DOI:10.1021/ja406532e