Switchable Fluorescent Imaging of Intracellular Telomerase Activity Using Telomerase-Responsive Mesoporous Silica Nanoparticle
This work designs a telomerase-responsive mesoporous silica nanoparticle (MSN) to realize in situ “off-on” imaging of intracellular telomerase activity. In the wrapping DNA (O1) sealed MSN probe, a black hole fluorescence quencher is covalently immobilized on the inner walls of the mesopores, while...
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| Published in | Journal of the American Chemical Society Vol. 135; no. 36; pp. 13282 - 13285 |
|---|---|
| Main Authors | , , |
| Format | Journal Article |
| Language | English |
| Published |
United States
American Chemical Society
11.09.2013
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| Subjects | |
| Online Access | Get full text |
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| Abstract | This work designs a telomerase-responsive mesoporous silica nanoparticle (MSN) to realize in situ “off-on” imaging of intracellular telomerase activity. In the wrapping DNA (O1) sealed MSN probe, a black hole fluorescence quencher is covalently immobilized on the inner walls of the mesopores, while fluorescein is loaded in the mesopores. In the presence of telomerase and dNTPs, the designed O1 can be extended and then moves away from the MSN surface via forming a rigid hairpin-like DNA structure. Thus the O1 can act as a “biogate” to block and release fluorescein for “off-on” switchable fluorescent imaging. The MSN probe exhibits good performance for sensitive in situ tracking of telomerase activity in living cells. The practicality of this protocol has been verified by monitoring the change of cellular telomerase activity in response to telomerase-related drugs. |
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| AbstractList | This work designs a telomerase-responsive mesoporous silica nanoparticle (MSN) to realize in situ "off-on" imaging of intracellular telomerase activity. In the wrapping DNA (O1) sealed MSN probe, a black hole fluorescence quencher is covalently immobilized on the inner walls of the mesopores, while fluorescein is loaded in the mesopores. In the presence of telomerase and dNTPs, the designed O1 can be extended and then moves away from the MSN surface via forming a rigid hairpin-like DNA structure. Thus the O1 can act as a "biogate" to block and release fluorescein for "off-on" switchable fluorescent imaging. The MSN probe exhibits good performance for sensitive in situ tracking of telomerase activity in living cells. The practicality of this protocol has been verified by monitoring the change of cellular telomerase activity in response to telomerase-related drugs. This work designs a telomerase-responsive mesoporous silica nanoparticle (MSN) to realize in situ "off-on" imaging of intracellular telomerase activity. In the wrapping DNA (O1) sealed MSN probe, a black hole fluorescence quencher is covalently immobilized on the inner walls of the mesopores, while fluorescein is loaded in the mesopores. In the presence of telomerase and dNTPs, the designed O1 can be extended and then moves away from the MSN surface via forming a rigid hairpin-like DNA structure. Thus the O1 can act as a "biogate" to block and release fluorescein for "off-on" switchable fluorescent imaging. The MSN probe exhibits good performance for sensitive in situ tracking of telomerase activity in living cells. The practicality of this protocol has been verified by monitoring the change of cellular telomerase activity in response to telomerase-related drugs.This work designs a telomerase-responsive mesoporous silica nanoparticle (MSN) to realize in situ "off-on" imaging of intracellular telomerase activity. In the wrapping DNA (O1) sealed MSN probe, a black hole fluorescence quencher is covalently immobilized on the inner walls of the mesopores, while fluorescein is loaded in the mesopores. In the presence of telomerase and dNTPs, the designed O1 can be extended and then moves away from the MSN surface via forming a rigid hairpin-like DNA structure. Thus the O1 can act as a "biogate" to block and release fluorescein for "off-on" switchable fluorescent imaging. The MSN probe exhibits good performance for sensitive in situ tracking of telomerase activity in living cells. The practicality of this protocol has been verified by monitoring the change of cellular telomerase activity in response to telomerase-related drugs. |
| Author | Qian, Ruocan Ding, Lin Ju, Huangxian |
| AuthorAffiliation | Nanjing University |
| AuthorAffiliation_xml | – name: Nanjing University |
| Author_xml | – sequence: 1 givenname: Ruocan surname: Qian fullname: Qian, Ruocan – sequence: 2 givenname: Lin surname: Ding fullname: Ding, Lin – sequence: 3 givenname: Huangxian surname: Ju fullname: Ju, Huangxian email: E-mail:hxju@nju.edu.cn |
| BackLink | https://www.ncbi.nlm.nih.gov/pubmed/23978191$$D View this record in MEDLINE/PubMed |
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| Snippet | This work designs a telomerase-responsive mesoporous silica nanoparticle (MSN) to realize in situ “off-on” imaging of intracellular telomerase activity. In the... This work designs a telomerase-responsive mesoporous silica nanoparticle (MSN) to realize in situ "off-on" imaging of intracellular telomerase activity. In the... |
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| SubjectTerms | DNA DNA - chemistry drugs fluorescein Fluorescence Fluorescent Dyes - chemistry HeLa Cells Humans image analysis monitoring nanoparticles Nanoparticles - chemistry Particle Size Porosity porous media silica Silicon Dioxide - chemistry standard operating procedures Surface Properties telomerase Telomerase - chemistry Telomerase - metabolism |
| Title | Switchable Fluorescent Imaging of Intracellular Telomerase Activity Using Telomerase-Responsive Mesoporous Silica Nanoparticle |
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