Gradient reversed-phase liquid chromatography of proteins on very short columns. Influence of particle size on peak dispersion

This paper is an experimental contribution to the behavior of proteins in gradient reversed-phase liquid chromatography. Very short columns and the smallest available particles were used (1 cm column length and 1-, 2-, 3-, 5-, and 10- mu m RO-Sil-C18-D particles). Phenones (small molecules) and prot...

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Bibliographic Details
Published inAnalytical chemistry (Washington) Vol. 60; no. 13; pp. 1329 - 1332
Main Authors Verzele, Maurice, Yang, Yang Bo, Dewaele, Chris, Berry, Vern
Format Journal Article
LanguageEnglish
Published Washington, DC American Chemical Society 01.07.1988
Subjects
Analytical chemistry
Chemistry
Chromatographic methods and physical methods associated with chromatography
Chromatography, Liquid
Exact sciences and technology
high-performance liquid chromatography
methodology
Other chromatographic methods
Particle Size
proteins
Proteins - analysis
Reversed phase chromatography
Particle size
Liquid chromatography
Chemical analysis
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Summary:This paper is an experimental contribution to the behavior of proteins in gradient reversed-phase liquid chromatography. Very short columns and the smallest available particles were used (1 cm column length and 1-, 2-, 3-, 5-, and 10- mu m RO-Sil-C18-D particles). Phenones (small molecules) and proteins (large molecules) were compared at different flow rates and different gradients. The decrease in peak dispersion with smaller particle size is more pronounced for proteins than for phenones.
Bibliography:istex:38C96F9669B3A1C170F08CF7E088964E8BFA6763
ark:/67375/TPS-4LVNWDX5-Q
ObjectType-Article-1
SourceType-Scholarly Journals-1
ObjectType-Feature-2
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ISSN:0003-2700
1520-6882
DOI:10.1021/ac00164a017