Bioinspired Self-Healing Hydrogel Based on Benzoxaborole-Catechol Dynamic Covalent Chemistry for 3D Cell Encapsulation

• Published in: ACS macro letters Vol. 7; no. 8; pp. 904 - 908
• Main Authors: Chen, Yangjun, Diaz-Dussan, Diana, Wu, Di, Wang, Wenda, Peng, Yi-Yang, Asha, Anika Benozir, Hall, Dennis G, Ishihara, Kazuhiko, Narain, Ravin
• Format: Journal Article
• Language: English
• Published: United States American Chemical Society 21.08.2018
• ISSN: 2161-1653; 2161-1653
• DOI: 10.1021/acsmacrolett.8b00434

Boronic ester, one typical example of dynamic covalent bonds, has presented great potential to prepare self-healing hydrogels. However, most of currently reported hydrogels based on boronic esters are formed at pH > 8, which impeded their further use in physiological conditions. In this study, we designed two kinds of zwitterionic copolymers with benzoxaborole and catechol pendant groups, respectively. Owing to the lower pK a value of benzoxaborole (7.2), gelation can happen easily at pH 7.4 PBS after mixing these two copolymers due to efficient formation of benzoxaborole-catechol complexations. The resulting hydrogels exhibited excellent self-healing property as well as dual pH/sugar responsiveness due to the dynamic nature of boronic ester. Moreover, benefiting from the cell membrane bioinspired 2-methacryloyloxyethyl phosphorylcholine (MPC)-based polymeric matrix, the hydrogel was further investigated for 3D cell encapsulation. The combination of biocompatible zwitterionic polymers with dynamic benzoxaborole-catechol complexation makes the hydrogels a promising platform for diverse potential bioapplications like drug delivery and tissue engineering.