High-resolution gas chromatographic/high-resolution mass spectrometric analysis of human serum on a whole-weight and lipid basis for 2,3,7,8-tetrachlorodibenzo-p-dioxin
The authors describe an analytical method to measure 2,3,7,8-tetrachlorodibenzo-p-dioxin (TCDD) and other TCDD isomers in human serum; the method uses a highly specific cleanup procedure and high-resolution gas chromatography/high-resolution mass spectrometry. The 2,3,7,8-TCDD is quantified by the i...
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Published in | Analytical chemistry (Washington) Vol. 59; no. 15; pp. 2000 - 2005 |
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Main Authors | , , , , , , |
Format | Journal Article |
Language | English |
Published |
Washington, DC
American Chemical Society
01.08.1987
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Subjects | |
Online Access | Get full text |
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Summary: | The authors describe an analytical method to measure 2,3,7,8-tetrachlorodibenzo-p-dioxin (TCDD) and other TCDD isomers in human serum; the method uses a highly specific cleanup procedure and high-resolution gas chromatography/high-resolution mass spectrometry. The 2,3,7,8-TCDD is quantified by the isotope dilution technique with ( super(13)C sub(12))-2,3,7,8-TCDD as the internal standard. Other TCDD isomers are estimated by using published relative response factors. The 1.25 part-per-quadrillion (ppq) limit of detection for 200-g samples is more than adequate for determining 2,3,7,8-TCDD concentrations in human serum specimens. The method is modified for analyzing 50-g and 10-g serum samples. Analytical accuracy is demonstrated by the results obtained in analyzing spiked samples. The method is unaffected by a number of potentially interfering compounds. |
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Bibliography: | istex:51A72C937880E0107EB7A6487435A4D83D1343B7 ark:/67375/TPS-KHMR5BZ7-C ObjectType-Article-2 SourceType-Scholarly Journals-1 ObjectType-Feature-1 content type line 23 ObjectType-Article-1 ObjectType-Feature-2 |
ISSN: | 0003-2700 1520-6882 |
DOI: | 10.1021/ac00142a023 |