Highly Selective Fluorogenic Multianalyte Biosensors Constructed via Enzyme-Catalyzed Coupling and Aggregation-Induced Emission

The development of a highly selective and fast responsive fluorogenic biosensor for diverse analytes ranging from bioactive small molecules to specific antigens is highly desirable but remains a considerable challenge. We herein propose a new approach by integrating substrate-selective enzymatic rea...

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Bibliographic Details
Published inJournal of the American Chemical Society Vol. 136; no. 28; pp. 9890 - 9893
Main Authors Wang, Xiaorui, Hu, Jinming, Zhang, Guoying, Liu, Shiyong
Format Journal Article
LanguageEnglish
Published WASHINGTON American Chemical Society 16.07.2014
Amer Chemical Soc
Subjects
antigens
Antigens - chemistry
Biosensing Techniques
biosensors
Carcinoembryonic Antigen - chemistry
Chemistry
Chemistry, Multidisciplinary
crosslinking
enzymatic reactions
enzyme-linked immunosorbent assay
fluorescence
Fluorescent Dyes - chemistry
glucose
Glucose - chemistry
Horseradish Peroxidase - chemistry
Humans
hydrogen peroxide
Hydrogen Peroxide - chemistry
Oxidation-Reduction
peroxidase
Physical Sciences
Reactive Oxygen Species - chemistry
Science & Technology
tyrosine
Tyrosine - chemistry
NANOPARTICLES
ORGANIC DOTS
CHEMISTRY
FLUORESCENT-PROBES
HYDROGEN-PEROXIDE
NANOSTRUCTURES
GENERATION
AIE
IN-SITU
LIVING CELLS
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Summary:The development of a highly selective and fast responsive fluorogenic biosensor for diverse analytes ranging from bioactive small molecules to specific antigens is highly desirable but remains a considerable challenge. We herein propose a new approach by integrating substrate-selective enzymatic reactions with fluorogens exhibiting aggregation-induced emission feature. Tyrosine-functionalized tetraphenylethene, TPE-Tyr, molecularly dissolves in aqueous media with negligible fluorescence emission; upon addition of horseradish peroxidase (HRP) and H2O2, effective cross-linking occurs due to HRP-catalyzed oxidative coupling of tyrosine moieties in TPE-Tyr. This leads to fluorescence emission turn-on and fast detection of H2O2 with high sensitivity and selectivity. As a validation of the new strategy’s generality, we further configure it into the biosensor design for glucose through cascade enzymatic reactions and for pathologically relevant antigens (e.g., human carcinoembryonic antigen) by combining with the ELISA kit.
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ISSN:0002-7863
1520-5126
1520-5126
DOI:10.1021/ja505278w