Imaging Laser-Induced Fluorescence Detection at the Taylor Cone of Electrospray Ionization Mass Spectrometry

Laser-induced fluorescence detection (LIF) is a powerful tool for the quantitative analysis of fluorescent molecules, widely used in glycan analysis with fluorophore labeled carbohydrates where each species has a common response factor. Electrospray ionization mass spectrometry (ESI-MS), on the othe...

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Published inAnalytical chemistry (Washington) Vol. 91; no. 12; pp. 7738 - 7743
Main Authors Szarka, Máte, Szigeti, Márton, Guttman, András
Format Journal Article
LanguageEnglish
Published United States American Chemical Society 18.06.2019
Subjects
Capillary electrophoresis
Carbohydrates
Chemistry
Electrophoresis
Electrospraying
Fluorescence
Glycan
Ionization
Ions
Laser induced fluorescence
Lasers
Mass spectrometry
Mass spectroscopy
Polysaccharides
Quantitation
Quantitative analysis
Scientific imaging
Spectroscopy
Sugar
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Summary:Laser-induced fluorescence detection (LIF) is a powerful tool for the quantitative analysis of fluorescent molecules, widely used in glycan analysis with fluorophore labeled carbohydrates where each species has a common response factor. Electrospray ionization mass spectrometry (ESI-MS), on the other hand, while revealing important structural information about individual analytes, generally can have different response factors for different species. For simpler and improved quantitation with ESI-MS, laser-induced fluorescent images were collected at the Taylor cone of the electrospray interface, enabling simultaneous and robust optical (quantitative) and MS (qualitative) detection of fluorophore labeled sugars. The performance of this universally applicable, interface design independent imaging laser-induced fluorescent (iLIF) system was demonstrated using capillary electrophoresis (CE)-ESI-MS in the analysis of aminopyrene-trisulfonate labeled linear maltooligosaccharides and branched glycans from human immunoglobulin. The limit of detection (LOD) of the iLIF system was in this case 40 attomole. The intra- and interday quantitative (peak area) reproducibilities of the system (RSD) were 4.15% and 6.79%, respectively.
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ISSN:0003-2700
1520-6882
DOI:10.1021/acs.analchem.9b01028