Solid-Substrate Fermentation of Corn Fiber by Phanerochaete chrysosporium and Subsequent Fermentation of Hydrolysate into Ethanol

The goal of this study was to develop a fungal process for ethanol production from corn fiber. Laboratory-scale solid-substrate fermentation was performed using the white-rot fungus Phanerochaete chrysosporium in 1 L polypropylene bottles as reactors via incubation at 37 °C for up to 3 days. Extrace...

Full description

Saved in:
Bibliographic Details
Published inJournal of agricultural and food chemistry Vol. 56; no. 11; pp. 3918 - 3924
Main Authors Shrestha, Prachand, Rasmussen, Mary, Khanal, Samir K, Pometto, Anthony L, van Leeuwen, J. (Hans)
Format Journal Article
LanguageEnglish
Published Washington, DC American Chemical Society 11.06.2008
Subjects
Online AccessGet full text

Cover

Loading…
More Information
Summary:The goal of this study was to develop a fungal process for ethanol production from corn fiber. Laboratory-scale solid-substrate fermentation was performed using the white-rot fungus Phanerochaete chrysosporium in 1 L polypropylene bottles as reactors via incubation at 37 °C for up to 3 days. Extracellular enzymes produced in situ by P. chrysosporium degraded lignin and enhanced saccharification of polysaccharides in corn fiber. The percentage biomass weight loss and Klason lignin reduction were 34 and 41%, respectively. Anaerobic incubation at 37 °C following 2 day incubation reduced the fungal sugar consumption and enhanced the in situ cellulolytic enzyme activities. Two days of aerobic solid-substrate fermentation of corn fiber with P. chrysosporium, followed by anaerobic static submerged-culture fermentation resulted in 1.7 g of ethanol/100 g of corn fiber in 6 days, whereas yeast (Saccharomyces cerevisiae) cocultured with P. chrysosporium demonstrated enhanced ethanol production of 3 g of ethanol/100 g of corn fiber. Specific enzyme activity assays suggested starch and hemi/cellulose contribution of fermentable sugar.
Bibliography:istex:42E02126F47F204FE49B2AF8367CD3E09E285B23
ark:/67375/TPS-R8MP3753-M
This research was funded by the Iowa Energy Center (IEC), Ames, IA (Grant 0404), The Cooperative State Research, Education, and Extension Service, U.S. Department of Agriculture (CSREES, USDA), under Agreement 2004-34188-15067, and the ISU Center for Crops Utilization Research.
ObjectType-Article-1
SourceType-Scholarly Journals-1
ObjectType-Feature-2
content type line 23
ISSN:0021-8561
1520-5118
DOI:10.1021/jf0728404