Rapid and Simultaneous Analysis of Free Sterols, Tocopherols, and Squalene in Edible Oils by Coupled Reversed-Phase Liquid Chromatography−Gas Chromatography

• Published in: Journal of agricultural and food chemistry Vol. 46; no. 4; pp. 1419 - 1422
• Main Authors: Villén, Jesús, Blanch, Gracia P, Ruiz del Castillo, María Luisa, Herraiz, Marta
• Format: Journal Article
• Language: English
• Published: Washington, DC American Chemical Society 01.04.1998
• Subjects: ANALYTICAL METHODS; Biological and medical sciences; DATA PROCESSING; Fat industries; FOOD ANALYSIS; Food industries; FOODS; Fundamental and applied biological sciences. Psychology; GAS CHROMATOGRAPHY; HPLC; OLIVE OIL; ON LINE; PLANT OILS; PROCESSING; RAPESEED OIL; RAPID METHODS; SAMPLING; SCREENING; STEROLS; TESTING; TOCOPHEROLS; TRITERPENOIDS; Squalene; Liquid chromatography; Olive oil; Edible oil; Gas chromatography; Reversed phase chromatography; Analysis method; Quality control; Simultaneous measurement; Tocopherol; Adulteration; Quantitative analysis; Sterol
• ISSN: 0021-8561; 1520-5118
• DOI: 10.1021/jf970706l

On-line coupling of reversed-phase liquid chromatography and gas capillary chromatography (RPLC-GC) using a programmed temperature vaporizer (PTV) as interface is used for the direct and simultaneous analysis of free sterols, tocopherols, and squalene of edible oils by direct injection (that is, without the need for prior enrichment of the sample). The proposed method allows large volume fractions of water methanol eluents to be transferred into the GC system, 2000 μL/min being the speed of sample introduction. The overall procedure including LC preseparation, transfer from LC to GC, and GC analysis takes <1 h. Keywords: On-line coupled RPLC-GC; sterols; tocopherols; squalene; direct analysis; edible oils