Genipin-Cross-Linked Layer-by-Layer Assemblies: Biocompatible Microenvironments To Direct Bone Cell Fate

• Published in: Biomacromolecules Vol. 15; no. 5; pp. 1602 - 1611
• Main Authors: Gaudière, Fabien, Morin-Grognet, Sandrine, Bidault, Laurent, Lembré, Pierre, Pauthe, Emmanuel, Vannier, Jean-Pierre, Atmani, Hassan, Ladam, Guy, Labat, Béatrice
• Format: Journal Article
• Language: English
• Published: Washington, DC American Chemical Society 12.05.2014
• Subjects: Applied sciences; Biocompatible Materials - chemical synthesis; Biocompatible Materials - chemistry; Biocompatible Materials - pharmacology; Bioengineering; Biological and medical sciences; Biomaterials; Cell Adhesion - drug effects; Cell Differentiation - drug effects; Cell Proliferation - drug effects; Cell Survival - drug effects; Cells, Cultured; Cellular Biology; Chemical Sciences; Chondroitin Sulfates - chemistry; Chondroitin Sulfates - pharmacology; Cross-Linking Reagents - chemical synthesis; Cross-Linking Reagents - chemistry; Cross-Linking Reagents - pharmacology; Exact sciences and technology; Fluorescence Resonance Energy Transfer; Humans; Iridoids - chemical synthesis; Iridoids - chemistry; Iridoids - pharmacology; Life Sciences; Medical sciences; Microscopy, Atomic Force; Natural polymers; Osteoblasts - cytology; Osteoblasts - drug effects; Osteogenesis - drug effects; Physicochemistry of polymers; Polymers; Quartz Crystal Microbalance Techniques; Spectroscopy, Fourier Transform Infrared; Starch and polysaccharides; Surgery (general aspects). Transplantations, organ and tissue grafts. Graft diseases; Technology. Biomaterials. Equipments; Self-assembled layer; Self assembly; Elastic modulus; Coating material; Aglycone; Mechanical properties; Multiple layer; Crosslinking; Semiinterpenetrating polymer network; Surface topography; Cell differentiation; Experimental study; Chemical reaction kinetics; Polyelectrolyte; Cell adhesion; Chondroitin sulfate A; Osteoblast; Glycosaminoglycan; Biomaterial; Manufacturing; Polyethylene imine
• ISSN: 1525-7797; 1526-4602
• DOI: 10.1021/bm401866w

The design of biomimetic coatings capable of improving the osseointegration of bone biomaterials is a current challenge in the field of bone repair. Toward this end, layer-by-layer (LbL) films composed of natural components are suitable candidates. Chondroitin sulfate A (CSA), a natural glycosaminoglycan (GAG), was used as the polyanionic component because it promotes osteoblast maturation in vivo. In their native state, GAG-containing LbL films are generally cytophobic because of their low stiffness. To stiffen our CSA-based LbL films, genipin (GnP) was used as a natural cross-linking agent, which is much less cytotoxic than conventional chemical cross-linkers. GnP-cross-linked films display an original combination of microscale topography and tunable mechanical properties. Structural characterization was partly based on a novel donor/acceptor Förster resonance energy transfer (FRET) couple, namely, FITC/GnP, which is a promising approach for further inspection of any GnP-cross-linked system. GnP-cross-linked films significantly promote adhesion, proliferation, and early and late differentiation of preosteoblasts.