Reproducible Two-Dimensional Capillary Electrophoresis Analysis of Barrett's Esophagus Tissues

• Published in: Analytical chemistry (Washington) Vol. 78; no. 17; pp. 5977 - 5986
• Main Authors: Kraly, James R, Jones, Megan R, Gomez, David G, Dickerson, Jane A, Harwood, Melissa M, Eggertson, Michael, Paulson, Thomas G, Sanchez, Carissa A, Odze, Robert, Feng, Ziding, Reid, Brian J, Dovichi, Norman J
• Format: Journal Article
• Language: English
• Published: Washington, DC American Chemical Society 01.09.2006
• Subjects: Amines - chemistry; Amines - metabolism; Analytical chemistry; Barrett Esophagus - metabolism; Barrett Esophagus - pathology; Biopsy; Cell Line; Chemistry; Chromatographic methods and physical methods associated with chromatography; Electrophoresis, Capillary - classification; Electrophoresis, Capillary - instrumentation; Electrophoresis, Capillary - methods; Epithelium - metabolism; Exact sciences and technology; General, instrumentation; Humans; Other chromatographic methods; Reproducibility of Results; Alanine; Fluorescence detector; Phenylalanine; Capillary electrophoresis; Micellar electrokinetic capillary chromatography; Fluorogenic reagent; Instrumentation; Barrett esophagus; Glycine; Protein; Tissue; Sensitivity; Reproducibility; Aminoacid; Biogenic amine; High speed; Quinoline; Two dimensional electrophoresis
• ISSN: 0003-2700; 1520-6882
• DOI: 10.1021/ac061029+

We have constructed a high-speed, two-dimensional capillary electrophoresis system with a compact and high-sensitivity fluorescence detector. This instrument is used for the rapid and reproducible separations of Barrett's esophagus tissue homogenates. Proteins and biogenic amines are labeled with the fluorogenic reagent 3-(2-furoyl)quinoline-2-carboxaldehyde. Labeled biomolecules are separated sequentially in two capillaries. The first capillary employs capillary sieving electrophoresis using a replaceable sieving matrix. Fractions are successively transferred to a second capillary where they undergo additional separation by micellar electrokinetic capillary chromatography. The comprehensive two-dimensional separation requires 60 min. Within-day migration time reproducibility is better than 1% in both dimensions for the 50 most intense features. Between-day migration time precision is 1.3% for CSE and better than 0.6% for MECC. Biopsies were obtained from the squamous epithelium in the proximal tubular esophagus, Barrett's epithelium from the distal esophagus, and fundus region of the stomach from each of three Barrett's esophagus patients with informed consent. We identified 18 features from the homogenate profiles as biogenic amines and amino acids. For each of the patients, Barrett's biopsies had more than 5 times the levels of phenylalanine and alanine as compared to squamous tissues. The patient with high-grade dysplasia shows the highest concentrations for 13 of the amino acids across all tissue types. Concentrations of glycine are 40 times higher in squamous biopsies compared to Barrett's and fundal biopsies from the patient with high-grade dysplasia. These results suggest that two-dimensional capillary electrophoresis may be of value for the rapid characterization of endoscopic and surgical biopsies.